三种酚类化合物对浮萍 G3 的影响。

Effects of three phenolic compounds onLemna gibba G3.

机构信息

Agricultural Research Service, U.S. Department of Agriculture, Foreign Disease-Weed Science Research, Bldg. 1301, 21701, Ft. Detrick, Frederick, Maryland.

出版信息

J Chem Ecol. 1988 Mar;14(3):845-53. doi: 10.1007/BF01018778.

DOI:10.1007/BF01018778

PMID:24276136

Abstract

Lemna gibba L. G3, (duckweed) was used as a bioassay organism to test the allelochemical effects of salicylic acid (SA), ferulic acid (FA), and umbelliferone (UM). Growth rate (K), dry weight (DW) and total chlorophyll (CHL) production were measured after seven days of growth. The bioassay procedure used 50 ml of E medium with and without sucrose in 125-ml Erlenmeyer flasks plus the selected concentration of allelochemical. At concentrations of 50 μM and greater, SA caused inhibition of K and DW production inL. gibba G3, while the threshold for CHL reduction was 20 μM. FA inhibited the DW and CHL production at 100 μM when the compound was auto-claved in E medium containing sucrose. Treatments of UM were least toxic with an inhibition threshold of 500 μM for K and DW production in medium without sucrose. UM did not reduce CHL production until 750 μM. In some cases, different thresholds of inhibition were observed depending on the presence or absence of sucrose and tartaric acid in the medium, and whether or not the chemicals were autoclaved with the medium.

摘要

浮萍（浮萍）被用作生物测定生物来测试水杨酸（SA）、阿魏酸（FA）和伞形酮（UM）的化感物质的影响。生长七天后测量生长速度（K）、干重（DW）和总叶绿素（CHL）的产生。生物测定程序使用 50ml 的 E 培养基，在 125 毫升的 Erlenmeyer 烧瓶中加入或不加入蔗糖，并加入选定浓度的化感物质。在 50μM 及更高浓度下，SA 导致浮萍 G3 的 K 和 DW 产生抑制，而 CHL 减少的阈值为 20μM。当 FA 在含有蔗糖的 E 培养基中自动灭菌时，在 100μM 时抑制 DW 和 CHL 的产生。UM 处理的毒性最小，在不含蔗糖的培养基中，K 和 DW 产生的抑制阈值为 500μM。UM 直到 750μM 才降低 CHL 的产生。在某些情况下，根据培养基中是否存在蔗糖和酒石酸以及化学物质是否与培养基一起高压灭菌，观察到不同的抑制阈值。