Shastri N, Gammon G, Miller A, Sercarz E E
J Exp Med. 1986 Sep 1;164(3):882-96. doi: 10.1084/jem.164.3.882.
Ak- or Ek-restricted T cells, generated by immunization with a 23-amino-acid peptide of hen egg-white lysozyme (amino acid 74-96), showed a strict correlation between the minimal peptide determinant recognized and the Ia molecule restricting recognition. All Ak-restricted clones obtained from six independently derived lines recognized determinants contained within peptides 74-86, while Ek-restricted clones recognized determinants within 85-96. This correlation was true whether B10.A mice (Ak, Ek) were immunized with peptide 74-96 or with each of the two smaller peptides (74-86 or 85-96). Furthermore, a T cell response could be obtained to peptide 74-86, but not to peptide 85-96 in B10.A (4R) mice, which express only the Ak molecule. Thus, an Ia molecule-associated selectivity exists in the choice of T cell determinants even within this small 23-amino-acid peptide antigen. Significant differences were noted, however, in the boundaries of the minimal peptide determinants recognized within peptide 74-96 by Ak- or Ek-restricted T cells, in comparison to those recognized by Ab-restricted T cells. These results indicate that interaction of the same peptide with distinct Ia molecules results in recognition of unique aspects of the antigenic determinants by the T cell receptor.
通过用鸡蛋清溶菌酶的23个氨基酸肽（氨基酸74 - 96）免疫产生的Ak或Ek限制性T细胞，在所识别的最小肽决定簇与限制识别的Ia分子之间显示出严格的相关性。从六个独立衍生系获得的所有Ak限制性克隆识别74 - 86肽内包含的决定簇，而Ek限制性克隆识别85 - 96内的决定簇。无论用肽74 - 96还是用两个较小的肽（74 - 86或85 - 96）对B10.A小鼠（Ak，Ek）进行免疫，这种相关性都是成立的。此外，在仅表达Ak分子的B10.A（4R）小鼠中，可以获得针对肽74 - 86的T细胞应答，但不能获得针对肽85 - 96的T细胞应答。因此，即使在这种小的23个氨基酸肽抗原内，在T细胞决定簇的选择中也存在Ia分子相关的选择性。然而，与Ab限制性T细胞识别的相比，Ak或Ek限制性T细胞在74 - 96肽内识别的最小肽决定簇的边界存在显著差异。这些结果表明，同一肽与不同的Ia分子相互作用导致T细胞受体识别抗原决定簇的独特方面。
