Department of Horticulture, University of Wisconsin Madison, Wisconsin, USA.
Theor Appl Genet. 1981 Mar;59(2):83-8. doi: 10.1007/BF00285895.
Charge and molecular weight heterogeneity of globulin-1 (G1) polypeptides of the bean, Phaseolus vulgaris L., were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Different bean cultivars were classified into three groups: 'Tendergreen', 'Sanilac', and 'Contender' on the basis of their protein subunit composition. Nine distinct major bands: α51,α49, α48.5,β48(T), β48(S), β47, γ45.5, γ45(S), and γ45(C), and two minor bands: γ46(T) and γ46(S) were found to account for the three profiles seen on one-dimensional SDS-PAGE. Two-dimensional analysis revealed these eleven protein bands to be composed of a minimum of fourteen distinct protein subunits. The 'Tendergreen' and 'Sanilac' types differ in their G1 polypeptide composition. The protein patterns of the 'Contender' types are intermediate, containing many protein subunits found in the patterns of the 'Tendergreen' and 'Sanilac' types suggesting a genetic and evolutionary relationship.
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)揭示了菜豆球蛋白-1(G1)多肽的电荷和分子量不均一性。根据其蛋白质亚基组成，不同的菜豆品种被分为三组：“Tendergreen”、“Sanilac”和“Contender”。在一维 SDS-PAGE 上观察到的三种图谱由 9 种不同的主要带：α51、α49、α48.5、β48(T)、β48(S)、β47、γ45.5、γ45(S)和γ45(C)和 2 种次要带：γ46(T)和γ46(S)组成。二维分析表明，这 11 条蛋白质带由至少 14 种不同的蛋白质亚基组成。“Tendergreen”和“Sanilac”两种类型在 G1 多肽组成上存在差异。“Contender”类型的蛋白质图谱是中间的，包含许多在“Tendergreen”和“Sanilac”类型的图谱中发现的蛋白质亚基，这表明它们之间存在遗传和进化关系。
