Gupta Neha, Shrivastava Nidhi, Singh Pramod Kumar, Bhagyawant Sameer S
School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, India.
Department of Bioscience & Biotechnology, Banasthali University, Banasthali 304022, India.
Biochem Res Int. 2016;2016:3136043. doi: 10.1155/2016/3136043. Epub 2016 Apr 30.
In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE) was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3-10 in a molecular weight range of 11-170 kDa. In both seed accessions maximum protein spots are seen in the pI range of 6-8.
在本研究中，对25份绿豆（Vigna aconitifolia）种子种质的植物化学成分进行了评估。这包括蛋白酶抑制剂、植酸、自由基清除活性和单宁。研究表明，这些植物化学成分的含量存在显著差异。光化学组成的存在与种子贮藏蛋白如白蛋白和球蛋白相关。使用二维凝胶电泳（2D-GE）对两个相关绿豆种质中总种子贮藏蛋白丰度进行了定性鉴定。在两份绿豆种质中，超过20个单个蛋白质组分以一系列斑点的形式分布在凝胶上。种子蛋白质组在11-170 kDa的分子量范围内，在3-10的广泛pI值范围内积累了高强度的斑点。在两份种子种质中，最大蛋白质斑点出现在pI为6-8的范围内。
