Kleiser C, Schneider J, Bayer H, Hunsmann G
J Gen Virol. 1986 Sep;67 ( Pt 9):1901-7. doi: 10.1099/0022-1317-67-9-1901.
Vaccines prepared from Friend leukaemia virus envelope and core polypeptides were compared for their efficiency in preventing erythroleukaemia in mice. High doses (100 micrograms) of gp85, the micellar complex of the envelope polypeptides gp70 and p15E, completely protected STU mice. The same dose of purified gp70 still protected about 80% of the animals, while p15E did not affect the cumulative mortality. The internal viral polypeptide p30 was ineffective. Serological examination indicated that immunity against death from leukaemia was mediated by specific antibodies. These leukaemia-preventing antibodies were predominantly induced by immunization with the gp70 env gene product, since p15E showed only minor protection. Glycoprotein gp70, however, was more effective when given as the gp85 micellar complex. An even more potent vaccine was obtained when gp70 was coupled to keyhole limpet haemocyanin (KLH) by glutaraldehyde. Ten micrograms gp70 coupled to KLH was enough to save more than 90% of Friend leukaemia virus-infected mice from erythroleukaemia. KLH may also be a suitable experimental carrier for subunits of gp70 or synthetic oligopeptides for viral vaccines.
对用弗氏白血病病毒包膜和核心多肽制备的疫苗预防小鼠红白血病的效率进行了比较。高剂量（100微克）的gp85（包膜多肽gp70和p15E的胶束复合物）能完全保护STU小鼠。相同剂量的纯化gp70仍能保护约80%的动物，而p15E对累积死亡率没有影响。内部病毒多肽p30无效。血清学检查表明，抗白血病死亡的免疫是由特异性抗体介导的。这些预防白血病的抗体主要由用gp70 env基因产物免疫诱导产生，因为p15E仅显示出轻微的保护作用。然而，糖蛋白gp70以gp85胶束复合物形式给药时更有效。当gp70通过戊二醛与钥孔血蓝蛋白（KLH）偶联时，获得了一种更有效的疫苗。10微克与KLH偶联的gp70足以使90%以上感染弗氏白血病病毒的小鼠免于患红白血病。KLH也可能是gp70亚基或病毒疫苗合成寡肽的合适实验载体。
