Britt W J, Collins J K, Chesebro B
J Exp Med. 1981 Sep 1;154(3):868-82. doi: 10.1084/jem.154.3.868.
Spleen cells from Rfv-3r/s mice with Friend virus-induced erythroleukemia were analyzed for expression of virus-induced proteins with monoclonal antiviral antibodies and conventional antisera. Leukemic spleen cells, 30-60 d after virus inoculation, expressed decreased amounts of ecotropic Friend murine leukemia helper virus gag- and env-encoded cell surface and intracellular proteins compared with leukemic cells tested 8-10 d after virus inoculation. In contrast, the spleen focus-forming virus-induced protein, gp55, was present on both leukemia cell populations. This difference appeared to be mediated by the humoral antibody response in Rfv-3r/s mice, which could recognize only ecotropic gag and env proteins, and not gp55. A new gp70 molecule cross-reactive with a recombinant Friend mink cell focus-inducing virus was found in large quantities on late leukemic cells. This protein appeared to be derived from a recombinant virus produced during the course of Friend virus infection. The appearance of this new gp70 suggests that recombinant viruses other than spleen focus-forming virus may play a role in Friend virus-induced erythroleukemia.
用单克隆抗病毒抗体和传统抗血清分析感染Friend病毒诱导的红白血病的Rfv-3r/s小鼠的脾细胞中病毒诱导蛋白的表达。与病毒接种后8-10天检测的白血病细胞相比,病毒接种后30-60天的白血病脾细胞中嗜异性Friend小鼠白血病辅助病毒gag和env编码的细胞表面及细胞内蛋白表达量减少。相反,脾灶形成病毒诱导的蛋白gp55在两个白血病细胞群体中均存在。这种差异似乎是由Rfv-3r/s小鼠的体液抗体反应介导的,该反应只能识别嗜异性gag和env蛋白,而不能识别gp55。在晚期白血病细胞上大量发现了一种与重组Friend貂细胞灶形成病毒交叉反应的新gp70分子。这种蛋白似乎来源于Friend病毒感染过程中产生的重组病毒。这种新gp70的出现表明,除脾灶形成病毒外的重组病毒可能在Friend病毒诱导的红白血病中起作用。