Department of Plant Pathology, University of Florida, 32611, Gainesville, FL, USA.
Plant Mol Biol. 1987 Mar;9(2):121-6. doi: 10.1007/BF00015644.
Polyspecific antibody to a 17 amino acid synthetic peptide from the maize T-cytoplasm urf13-T mitochondrial open reading frame immunoprecipitated a 13 kD polypeptide from (35)S-methionine incorporations of T cytoplasm maize. Male-fertile, toxin-insensitive mutants in which the urf13-T sequence is deleted do not synthesize the 13 kD polypeptide. A mutant designated T-4, which carries a 5 bp insertion and a premature stop codon, synthesizes a truncated polypeptide, corresponding to an open reading frame of 8.3 kD. Thus the 13 kD polypeptide is trunctated or absent in mutants expressing male fertility and toxin insensitivity in T-cytoplasm maize.
多特异性抗体针对来自玉米 T 细胞质 urf13-T 线粒体开放阅读框的 17 个氨基酸合成肽，从（35）S-蛋氨酸掺入 T 细胞质玉米中免疫沉淀出 13 kD 多肽。urf13-T 序列缺失的雄性可育、毒素不敏感突变体不合成 13 kD 多肽。一个被命名为 T-4 的突变体携带 5 个碱基对的插入和一个过早的终止密码子，合成一个截断的多肽，对应于 8.3 kD 的开放阅读框。因此，在表达雄性育性和毒素不敏感性的 T 细胞质玉米突变体中，13 kD 多肽被截断或缺失。
