Liu H T, Baserga R, Mercer W E
Mol Cell Biol. 1985 Nov;5(11):2936-42. doi: 10.1128/mcb.5.11.2936-2942.1985.
We have studied a panel of 10 genes and cDNA sequences that are expressed in a cell cycle-dependent manner in different types of cells from different species and that are inducible by different mitogens. These include five sequences (c-myc, 4F1, 2F1, 2A9, and KC-1) that are preferentially expressed in the early part of the G1 phase, three genes (ornithine decarboxylase, p53, and c-rasHa) preferentially expressed in middle or late G1, and two genes (thymidine kinase and histone H3) preferentially expressed in the S phase of the cell cycle. We have studied the expression of these genes in nonpermissive (tsAF8) and semipermissive (Swiss 3T3) cells infected with adenovirus type 2. Under the conditions of these experiments, adenovirus type 2 infection stimulates cellular DNA synthesis in both tsAF8 and 3T3 cells. However, four of the five early G1 genes (c-myc, 4F1, KC-1, and 2A9) and one of the late G1 genes (c-ras) are not induced by adenovirus infection, although they are strongly induced by serum. The other sequences (2F1, ornithine decarboxylase, p53, thymidine kinase, and histone H3) are activated by both adenovirus and serum. We conclude that the cell cycle-dependent genes activated by adenovirus 2 are a subset of the cell cycle-dependent genes activated by serum. The data suggest that the mechanisms by which serum and adenovirus induce cellular DNA synthesis are not identical.
我们研究了一组10个基因和cDNA序列,它们在来自不同物种的不同类型细胞中以细胞周期依赖性方式表达,并且可被不同的促有丝分裂原诱导。这些包括五个在G1期早期优先表达的序列(c-myc、4F1、2F1、2A9和KC-1),三个在G1期中期或后期优先表达的基因(鸟氨酸脱羧酶、p53和c-rasHa),以及两个在细胞周期S期优先表达的基因(胸苷激酶和组蛋白H3)。我们研究了这些基因在感染2型腺病毒的非允许(tsAF8)和半允许(瑞士3T3)细胞中的表达。在这些实验条件下,2型腺病毒感染刺激tsAF8和3T3细胞中的细胞DNA合成。然而,五个早期G1基因中的四个(c-myc、4F1、KC-1和2A9)和一个晚期G1基因(c-ras)不会被腺病毒感染诱导,尽管它们会被血清强烈诱导。其他序列(2F1、鸟氨酸脱羧酶、p53、胸苷激酶和组蛋白H3)会被腺病毒和血清激活。我们得出结论,2型腺病毒激活的细胞周期依赖性基因是血清激活的细胞周期依赖性基因的一个子集。数据表明血清和腺病毒诱导细胞DNA合成的机制并不相同。