Wu Jiang, Shao Sijia, Zhou Fan, Wen Siyuan, Chen Fu, Han Xiaodong
Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210093, China.
Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing 210093, China; State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, 210093, China.
Environ Toxicol Pharmacol. 2014 Jan;37(1):1-6. doi: 10.1016/j.etap.2013.10.012. Epub 2013 Oct 28.
There are increasing concerns about health risk of human exposure to microcystin-LR (MC-LR) over the past few decades. Nevertheless, its female mammalian reproductive toxicity has not been addressed yet. In the present study, we firstly reported MC-LR could impact female reproductive function of mammals. After 28 days MC-LR exposure, relative ovary weight significantly reduced in 20 μg/kg MC-LR group and this reduction may be relative with pathomorphological changes of ovary. The result of histological evaluation of follicles showed that primordial follicles decreased roughly in half at high dose level compared with control. Since serum hormone assay indicated that MC-LR induced decrease of progesterone but not FSH or LH, disturbance of estrus cycle was seemed to result from direct impact of ovary rather than indirectly from hypothalamus or pituitary. As expected, MC-LR was detected in the ovaries of MC-LR exposure mice by immunoblot analysis.
在过去几十年里，人们越来越关注人类接触微囊藻毒素-LR（MC-LR）对健康的风险。然而，其对雌性哺乳动物的生殖毒性尚未得到研究。在本研究中，我们首次报道MC-LR会影响哺乳动物的雌性生殖功能。MC-LR暴露28天后，20μg/kg MC-LR组的相对卵巢重量显著降低，这种降低可能与卵巢的病理形态学变化有关。卵泡组织学评估结果显示，与对照组相比，高剂量组的原始卵泡数量大致减少了一半。由于血清激素检测表明MC-LR导致孕酮水平下降，但不影响促卵泡生成素（FSH）或促黄体生成素（LH），发情周期紊乱似乎是由MC-LR对卵巢的直接影响导致的，而非间接通过下丘脑或垂体。正如预期的那样，通过免疫印迹分析在暴露于MC-LR的小鼠卵巢中检测到了MC-LR。
