人脐带间充质干细胞通过调节实验性结肠炎中的 Treg/Th17 表型下调炎症反应。
Human umbilical cord-derived mesenchymal stem cells downregulate inflammatory responses by shifting the Treg/Th17 profile in experimental colitis.
机构信息
Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, China.
出版信息
Pharmacology. 2013;92(5-6):257-64. doi: 10.1159/000354883. Epub 2013 Nov 20.
BACKGROUND/AIMS: The aim of this study was to evaluate the effect and mechanisms of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on immune responses in murine colitis.
METHODS
Mice with dextran sulfate sodium (DSS)-induced colitis were injected intraperitoneally with hUC-MSCs or human bone marrow-derived MSCs. The cytokine levels from lamina propria mononuclear cells (LPMCs) and colon tissue were measured using ELISA. Treg and Th17 cells were analyzed using flow cytometry. The proliferation of LPMCs was assessed using Cell Counting Kit-8.
RESULTS
hUC-MSCs ameliorate DSS-induced colitis via the downregulation of colon inflammatory responses. Furthermore, hUC-MSCs adjusted modulation of Treg/Th17 cells in the spleen and mesenteric lymph nodes. hUC-MSCs also inhibited LPMCs in vitro.
CONCLUSION
hUC-MSCs may be an alternative source of stem cells and are worthy of study in long-term clinical trials.
背景/目的：本研究旨在评估人脐带间充质干细胞（hUC-MSCs）对小鼠结肠炎免疫反应的影响及其机制。
方法
采用葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型，通过腹腔注射 hUC-MSCs 或人骨髓间充质干细胞（hBM-MSCs）进行干预。采用 ELISA 法检测肠固有层单核细胞（LPMCs）和结肠组织中的细胞因子水平。采用流式细胞术分析 Treg 和 Th17 细胞。采用 Cell Counting Kit-8 检测 LPMCs 的增殖情况。
结果
hUC-MSCs 通过下调结肠炎症反应来改善 DSS 诱导的结肠炎。此外，hUC-MSCs 还调节了脾脏和肠系膜淋巴结中 Treg/Th17 细胞的平衡。hUC-MSCs 还能抑制体外的 LPMCs。
结论
hUC-MSCs 可能是一种替代的干细胞来源，值得进行长期临床试验研究。
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