Frank Reidy Research Center for Bioelectrics/Old Dominion University 4211 Monarch Way, Suite 300, Norfolk, Virginia 23508, USA.
Cancers (Basel). 2010 Sep 27;2(3):1731-70. doi: 10.3390/cancers2031731.
Two new cancer therapies apply bioelectric principles. These methods target tumor structures locally and function by applying millisecond electric fields to deliver plasmid DNA encoding cytokines using electrogene transfer (EGT) or by applying rapid rise-time nanosecond pulsed electric fields (nsPEFs). EGT has been used to locally deliver cytokines such as IL-12 to activate an immune response, resulting in bystander effects. NsPEFs locally induce apoptosis-like effects and affect vascular networks, both promoting tumor demise and restoration of normal vascular homeostasis. EGT with IL-12 is in melanoma clinical trials and nsPEFs are used in models with B16F10 melanoma in vitro and in mice. Applications of bioelectrics, using conventional electroporation and extensions of it, provide effective alternative therapies for melanoma.
两种新的癌症疗法应用了生物电原理。这些方法通过应用毫秒级电场来局部靶向肿瘤结构，使用电基因转移（EGT）传递编码细胞因子的质粒 DNA，或应用快速上升时间纳秒级脉冲电场（nsPEFs）来发挥作用。EGT 已被用于局部递送达 IL-12 等细胞因子，以激活免疫反应，从而产生旁观者效应。nsPEFs 局部诱导类似凋亡的效应并影响血管网络，这两者都促进肿瘤死亡和正常血管动态平衡的恢复。IL-12 的 EGT 正在黑色素瘤临床试验中进行，nsPEFs 则用于体外的 B16F10 黑色素瘤模型和小鼠模型中。使用传统电穿孔及其扩展的生物电学应用为黑色素瘤提供了有效的替代疗法。
