Beham A, Weybora W, Lackinger E, Denk H, Björklund V, Björklund B
Virchows Arch A Pathol Anat Histopathol. 1986;409(5):641-55. doi: 10.1007/BF00713430.
The presence and distribution of tissue polypeptide antigen (TPA) were assessed in gastrointestinal carcinomas of different origin, morphology and degree of differentiation. Immunocytochemistry was employed, using the PAP technique on formalin-fixed, paraffin-embedded material and compared with the results obtained with antibodies to cytokeratins. Like cytokeratins, TPA was a reliable marker of epithelial differentiation and showed tissue distribution patterns similar to cytokeratins, as revealed by antibodies with broad-range cytokeratin immunoreactivity. In most carcinomas, TPA-specific immunostaining was less intense than in non-neoplastic tissue. No direct relationship between intensity of TPA staining and morphological degree of differentiation and proliferation was found. TPA staining was most pronounced at the periphery of the cells. In stratified epithelium, i.e. oesophageal mucosa, basally located cells exceeded superficial cells in TPA immunoreactivity in contrast to the cytokeratin antibodies which decorated the more superficially placed cell layers. TPA and cytokeratin staining patterns were similar in neoplastic and non-neoplastic gastric, intestinal mucosa, as well as in biliary tract epithelium. Antral and cardial mucoid glands of the stomach as well as gastric carcinomas of the pylorocardial type remained unstained with both types of antibodies. Similar staining with TPA and cytokeratin antibodies was also observed in pancreatic and liver tissue. In this study, hepatocytes were, although weakly, stained by TPA antibodies and an identical staining was found with benign and malignant hepatocellular neoplasms. Ductal and ductular TPA-staining was most conspicuous and so was the immunoreactivity of cholangiocellular carcinomas. A comparison between TPA and cytokeratins was also made by immunoblotting which revealed immunoreactivity of antibodies to TPA with cytokeratin polypeptides of different species (man, mouse) and organs (epidermis, liver), particularly with the cytokeratin component 8 of human liver and the related component A of mouse liver. The significance of this finding is uncertain until the pertinent epitopes have been revealed by monoclonal mapping of the components which exhibit similar molecular weights by SDS polyacrylamide gel electrophoresis.
对不同起源、形态和分化程度的胃肠道癌组织中的组织多肽抗原（TPA）的存在及分布情况进行了评估。采用免疫细胞化学方法，对福尔马林固定、石蜡包埋的材料运用PAP技术，并与细胞角蛋白抗体的检测结果进行比较。与细胞角蛋白一样，TPA是上皮分化的可靠标志物，且呈现出与细胞角蛋白相似的组织分布模式，这一点通过具有广泛细胞角蛋白免疫反应性的抗体得以揭示。在大多数癌组织中，TPA特异性免疫染色强度低于非肿瘤组织。未发现TPA染色强度与形态学分化程度及增殖之间存在直接关系。TPA染色在细胞周边最为明显。在复层上皮，即食管黏膜中，位于基底的细胞TPA免疫反应性超过表层细胞，这与装饰表层细胞层的细胞角蛋白抗体情况相反。在肿瘤性和非肿瘤性胃、肠黏膜以及胆管上皮中，TPA和细胞角蛋白染色模式相似。胃的胃窦和贲门黏液腺以及幽门心型胃癌对这两种抗体均无染色。在胰腺和肝组织中也观察到TPA和细胞角蛋白抗体的相似染色情况。在本研究中，肝细胞虽被TPA抗体弱阳性染色，且在良性和恶性肝细胞肿瘤中发现相同的染色情况。导管和小导管的TPA染色最为明显，胆管细胞癌的免疫反应性也是如此。还通过免疫印迹法对TPA和细胞角蛋白进行了比较，结果显示TPA抗体与不同物种（人、小鼠）和器官（表皮、肝脏）的细胞角蛋白多肽具有免疫反应性，特别是与人肝脏的细胞角蛋白成分8和小鼠肝脏的相关成分A有反应。在通过SDS聚丙烯酰胺凝胶电泳显示分子量相似的成分的单克隆定位揭示相关表位之前，这一发现的意义尚不确定。
