Moll R, Levy R, Czernobilsky B, Hohlweg-Majert P, Dallenbach-Hellweg G, Franke W W
Lab Invest. 1983 Nov;49(5):599-610.
Cytokeratins are a family of polypeptides of intermediate filaments which in diverse epithelia are expressed in different, yet specific, combinations. We have studied the cytokeratins present in normal epithelia of the female genital tract, in comparison with those present in genital tract carcinomas, by two-dimensional gel electrophoresis of cytoskeletal proteins from microdissected tissues and by immunofluorescence microscopy. Cells of ovarian mesothelium, oviduct, endometrium, and endocervix contain cytokeratin polypeptides nos. 7, 8, 18, and 19. By contrast, tonofilaments of the stratified squamous epithelia of vagina and exocervix contain cytokeratins 4, 5, 6, 13, 14, 15, 16, and 19. Exocervical regions distant from the endo-exocervical junction as well as vagina contain, in addition, the large (Mr 68,000) and basic cytokeratin component no. 1, previously described in epidermis. Endocervical squamous metaplasia at the endo-exocervical border displays a complex cytokeratin pattern, probably due to cell-type heterogeneity. Similar cytokeratin patterns are also observed in genital tract epithelia of the cow and mouse. In human carcinomas of the female genital tract, two main types of cytokeratin patterns can be distinguished. Ovarian carcinomas and endometrial adenocarcinomas express cytokeratins 7, 8, 18, and 19 and, thus, maintain the pattern of the cells of their origin. In endocervical adenocarcinomas the additional presence of component no. 17 has been noted. Nonkeratinizing squamous cell carcinomas of the cervix show a very complex pattern (cytokeratins 5, 6, 7, 8, 13, 14, 15, 17, 18, and 19). Keratinizing squamous cell carcinomas of the cervix display lower complexity and lack cytokeratins 7, 8, and 18. When frozen sections are examined by immunofluorescence microscopy, all epithelia of the genital tract are stained with the monoclonal cytokeratin antibody KG 8.13. Simple epithelia but not the stratified epithelia of vagina and exocervix also react with monoclonal antibodies specific for cytokeratins 8 or 18. The value of cytokeratin polypeptide patterns in distinguishing diverse epithelial cell types of the female genital tract, in elucidating the histogenesis of neoplasms, and in providing a new tool for the differential diagnosis of tumors is discussed.
细胞角蛋白是中间丝的多肽家族,在不同上皮组织中以不同但特定的组合表达。我们通过对显微切割组织的细胞骨架蛋白进行二维凝胶电泳以及免疫荧光显微镜检查,研究了女性生殖道正常上皮组织中存在的细胞角蛋白,并与生殖道癌中的细胞角蛋白进行了比较。卵巢间皮、输卵管、子宫内膜和子宫颈内膜的细胞含有细胞角蛋白多肽7、8、18和19。相比之下,阴道和子宫颈外口的复层鳞状上皮的张力丝含有细胞角蛋白4、5、6、13、14、15、16和19。此外,远离子宫颈内-外口交界处的子宫颈外口区域以及阴道还含有先前在表皮中描述的大分子量(68,000)碱性细胞角蛋白成分1。子宫颈内-外口交界处的子宫颈鳞状化生显示出复杂的细胞角蛋白模式,可能是由于细胞类型的异质性。在牛和小鼠的生殖道上皮中也观察到类似的细胞角蛋白模式。在女性生殖道癌中,可以区分出两种主要的细胞角蛋白模式。卵巢癌和子宫内膜腺癌表达细胞角蛋白7、8、18和19,因此保持了其起源细胞的模式。在子宫颈腺癌中,已注意到还存在成分17。子宫颈非角化鳞状细胞癌显示出非常复杂的模式(细胞角蛋白5、6、7、8、13、14、15、17、18和19)。子宫颈角化鳞状细胞癌的复杂性较低,并且缺乏细胞角蛋白7、8和18。当通过免疫荧光显微镜检查冰冻切片时,生殖道的所有上皮都用单克隆细胞角蛋白抗体KG 8.13染色。简单上皮而非阴道和子宫颈外口的复层上皮也与针对细胞角蛋白8或18的单克隆抗体发生反应。本文讨论了细胞角蛋白多肽模式在区分女性生殖道不同上皮细胞类型、阐明肿瘤组织发生以及为肿瘤鉴别诊断提供新工具方面的价值。