Los Angeles, Calif. From the University of Southern California Keck School of Medicine; the John Wayne Cancer Center; and Cedars-Sinai Medical Center.
Plast Reconstr Surg. 2013 Dec;132(6):932e-939e. doi: 10.1097/PRS.0b013e3182a80652.
Supplementation of fat grafts with stromal vascular fraction cells is an emerging technique used to improve graft reliability. A variety of systems for isolating stromal vascular fraction cells are commercially available. The lack of performance data obtained operating the systems in a standardized environment prevents objective assessment of performance. This prospective, blinded study compared performance of four commercially available stromal vascular fraction isolation systems when operated in a clinical outpatient surgery environment.
Four different systems were compared: (1) PNC's Multi Station, (2) CHA Biotech Cha-Station, (3) Cytori Celution 800/CRS System, and (4) Medi-Khan's Lipokit with MaxStem. Identical lipoaspirate samples from five separate volunteer donors were used to evaluate system process time, viable cell yield, composition, residual enzyme, and operating costs.
The mean processing time ranged from 88 to 115 minutes. The highest mean number of viable nucleated cells was obtained using the Celution System (2.41 × 10 cells/g) followed by the Multi Station (1.07 × 10 cells/g). Lipokit and Cha-Station systems yielded nearly a log fewer nucleated cells (0.35 × 10 cells/g and 0.05 × 10 cells/g, respectively). The Celution System also yielded significantly more endothelial cells, CD34/CD31 cells, and adipose-derived stem cells (colony-forming unit-fibroblast). Residual enzyme levels observed with the Multi Station, Cha-Station, and Lipokit, respectively, averaged 5.1-, 13.0-, and 57-fold higher than that observed with the Celution System.
Although all systems generated measurable amounts of stromal vascular fraction, significant variability exists in the number, identity, and safety profiles of recovered viable cells. Side-by-side clinical trials will be required to establish the relevance of these differences.
脂肪移植物中基质血管成分细胞的补充是一种新兴的技术，用于提高移植物的可靠性。有多种商业上可获得的系统用于分离基质血管成分细胞。由于缺乏在标准化环境中操作这些系统获得的性能数据，因此无法客观评估性能。这项前瞻性、盲法研究比较了四种市售的基质血管成分分离系统在临床门诊手术环境中操作时的性能。
比较了四种不同的系统：（1）PNC 的多站系统，（2）CHA Biotech Cha-Station，（3）Cytori Celution 800/CRS 系统，（4）Medi-Khan 的 Lipokit with MaxStem。使用相同的脂肪抽吸样本，从五名不同的志愿者中评估系统处理时间、活细胞产量、组成、残留酶和运营成本。
平均处理时间范围为 88 至 115 分钟。使用 Celution 系统获得的活核细胞数量最高（2.41×10^6 个细胞/g），其次是多站系统（1.07×10^6 个细胞/g）。Lipokit 和 Cha-Station 系统产生的核细胞数量几乎少了一个对数级（分别为 0.35×10^6 个细胞/g 和 0.05×10^6 个细胞/g）。Celution 系统还产生了显著更多的内皮细胞、CD34/CD31 细胞和脂肪源性干细胞（集落形成单位-成纤维细胞）。观察到的 Multi Station、Cha-Station 和 Lipokit 的残留酶水平分别比 Celution 系统高 5.1、13.0 和 57 倍。
尽管所有系统都产生了可测量数量的基质血管成分，但回收的活细胞的数量、身份和安全性特征存在显著差异。需要进行并列临床试验才能确定这些差异的相关性。
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