van de Molengraft F, Ramaekers F, Jap P, Vooijs P, Mungyer G
Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;51(4):285-301. doi: 10.1007/BF02899038.
Hepatocellular carcinoma cells obtained from ascitic fluid after diethylnitrosamine treatment of Sewall Wright strain-2 guinea pigs produce solid (primary) tumors, lymph-node metastases and malignant ascites when reinjected into animals of the same strain. When brought into culture the cells settle, form multilayer cultures and can be maintained in passage. In addition to epithelium-specific cytokeratin intermediate filaments (IF), these latter cells, like most cultured cells, also contain vimentin. Hepatocellular carcinoma cells in solid tumors and in metastatic tumors retain their original keratin IF and in general do not have an additional vimentin-IF system. When the tumor cells are present in ascites they develop vimentin-IF in addition to cytokeratin filaments. Vimentin is gradually lost when these cells sediment onto the peritoneal surface and proliferate continuously to form papillary projections, or when they are detected as circumscribed metastases. It seems likely, therefore, that in this system the synthesis of an additional vimentin cytoskeleton is related to reduced cell-to-cell contact and to the ability of the cells to survive individually or as cell clusters in body fluids, without being part of a cohesive tissue.
用二乙基亚硝胺处理斯维尔·赖特2号品系豚鼠后，从其腹水中获取的肝癌细胞，重新注射到同品系动物体内时，会产生实体（原发性）肿瘤、淋巴结转移和恶性腹水。当这些细胞进行培养时，它们会贴壁，形成多层培养物，并能传代培养。除了上皮特异性细胞角蛋白中间丝（IF）外，这些细胞与大多数培养细胞一样，还含有波形蛋白。实体瘤和转移瘤中的肝癌细胞保留其原始的角蛋白IF，一般没有额外的波形蛋白IF系统。当肿瘤细胞存在于腹水中时，除了细胞角蛋白丝外，它们还会形成波形蛋白IF。当这些细胞沉积到腹膜表面并持续增殖形成乳头状突起时，或者当它们被检测为局限性转移时，波形蛋白会逐渐丢失。因此，在这个系统中，额外的波形蛋白细胞骨架的合成似乎与细胞间接触减少以及细胞在体液中单独或作为细胞簇存活的能力有关，而不是作为紧密组织的一部分。
