School of Civil and Environmental Engineering, Yonsei University, Seoul, South Korea,
Environ Sci Pollut Res Int. 2014 Mar;21(5):3722-32. doi: 10.1007/s11356-013-2366-6. Epub 2013 Nov 27.
Successive alkalinity-producing systems (SAPSs) is a key unit process in the passive treatment of acidic mine drainage. Physico-chemistry and pyrosequencing-based bacterial communities of two passive treatment processes in Gapjung (GJ) and Seokbong (SB) were analyzed. The influent of SB harbored higher levels of acidity and metals than that of GJ. SAPS-SB demonstrated better performance of acidity neutralization and metal removal than SAPS-GJ, despite its shorter hydraulic retention time and higher acidity. System diagnosis revealed that the capacities of SAPSs were not well predicted in the design steps. Bacterial diversity indices and composition were compared at the same sequence read number for fair evaluation. Most of the bacterial sequences were affiliated with uncultured species. A notable difference was observed in the bacterial community compositions of the SAPSs in GJ and SB. Classes of putative sulfate-reducing bacteria, Clostridia (8.3 %) and Deltaproteobacteria (6.1 %), were detected in SAPS-GJ, and Clostridia (14.6 %) was detected in SAPS-SB. Bacilli, which is not a known sulfate-reducing bacterial group, was the second largest class (12.8 %) in SAPS-GJ and the largest class (51.1 %) in SAPS-SB, suggesting that Bacilli may have a prominent role in SAPS. One hundred ninety operational taxonomic units were shared, which occupied ~10 % of each number of total operational taxonomic units in SAPS-GJ and SAPS-SB, respectively. Bacilli and Clostridia were the major shared classes, and Bacillus, Lysinibacillus, and Ureibacillus were the major shared genera. Rarefaction analysis, richness estimates, diversity estimates, and abundance rank analysis show that the sediment bacterial community of SAPS-GJ was more diverse and more evenly distributed than that of SAPS-SB.
连续产碱系统（SAPSs）是酸性矿山排水被动处理的关键单元过程。分析了嘉平（GJ）和朔邦（SB）两个被动处理过程的物理化学性质和基于焦磷酸测序的细菌群落。SB 的进水酸度和金属含量均高于 GJ。尽管 SAPS-SB 的水力停留时间较短，酸度较高，但它在中和酸度和去除金属方面的性能优于 SAPS-GJ。系统诊断表明，在设计步骤中 SAPS 的容量无法很好地预测。为了公平评估，在相同的序列读长下比较了细菌多样性指数和组成。大多数细菌序列与未培养物种有关。在 GJ 和 SB 的 SAPS 中观察到细菌群落组成的显著差异。在 SAPS-GJ 中检测到假定的硫酸盐还原菌纲 Clostridia（8.3%）和 Deltaproteobacteria（6.1%），在 SAPS-SB 中检测到 Clostridia（14.6%）。芽孢杆菌不是已知的硫酸盐还原细菌群，但在 SAPS-GJ 中是第二大分类（12.8%），在 SAPS-SB 中是最大的分类（51.1%），这表明芽孢杆菌可能在 SAPS 中具有重要作用。有 190 个操作分类单元（OTUs）被共享，分别占 SAPS-GJ 和 SAPS-SB 中总 OTUs 数量的~10%。芽孢杆菌和梭菌是主要的共享分类，芽孢杆菌、Lysinibacillus 和 Ureibacillus 是主要的共享属。稀疏分析、丰富度估计、多样性估计和丰度等级分析表明，SAPS-GJ 的沉积物细菌群落比 SAPS-SB 更具多样性，分布更均匀。
