Tunik Selcuk, Ayaz Ercan, Akpolat Veysi, Nergiz Yusuf, Isen Kenan, Celik M Salih, Seker Ugur
Department of Histology and Embryology, Faculty of Medicine, University of Dicle, 21280 Diyarbakir, Turkey.
Anal Quant Cytopathol Histpathol. 2013 Oct;35(5):253-60.
To investigate the role of extremely low frequency pulsed and sinusoidal electromagnetic fields on kidney tissues.
Twenty-seven male Wistar albino rats were used. The rats were divided into 3 groups (n = 9): control group, sinusoidal electromagnetic field (SEMF) group, and pulsed electromagnetic field (PEMF) group. The SEMF and PEMF groups (pulse time 25 microsn, pulse frequency 50 Hz) were subjected to 1.5 mT, 50 Hz, exposure 6 hours a day, 5 days a week for 28 days in methacrylate boxes. Formalin-fixed, paraffin-embedded kidney tissue sections were stained with hematoxylin-eosin, Gomori and periodic acid-Schiff. In addition, matrix metalloproteinase-2 (MMP-2) and 9 (MMP-9), E-cadherin and collagen type IV expression levels were examined immunohistochemically.
Thickening of glomerular basement membranes was evident in electromagnetic fields, especially in the SEMF group. In addition, expression levels of E-cadherin were decreased with electromagnetic field (EMF) exposure. The expression level of MMP-9 increased, and MMP-2 and collagen type IV expression levels were not altered with EMF exposure.
Both EMFs changed the molecular component of the kidney adversely.
探讨极低频脉冲和正弦电磁场对肾脏组织的作用。
使用27只雄性Wistar白化大鼠。将大鼠分为3组（n = 9）：对照组、正弦电磁场（SEMF）组和脉冲电磁场（PEMF）组。SEMF组和PEMF组（脉冲时间25微秒，脉冲频率50赫兹）置于甲基丙烯酸酯盒中，每天暴露6小时，每周5天，持续28天，暴露强度为1.5毫特斯拉，频率50赫兹。用苏木精-伊红、Gomori和过碘酸-希夫对福尔马林固定、石蜡包埋的肾脏组织切片进行染色。此外，采用免疫组织化学法检测基质金属蛋白酶-2（MMP-2）和9（MMP-9）、E-钙黏蛋白和IV型胶原的表达水平。
在电磁场中，尤其是SEMF组，肾小球基底膜明显增厚。此外，随着电磁场（EMF）暴露，E-钙黏蛋白的表达水平降低。MMP-9的表达水平升高，而MMP-2和IV型胶原的表达水平在EMF暴露后未发生改变。
两种电磁场均对肾脏的分子成分产生了不利影响。
