Zhu Huang, Wang Jie, Cui Jiefeng, Fan Xianqun
Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Toxicol Ind Health. 2016 Jun;32(6):1042-51. doi: 10.1177/0748233714545837. Epub 2014 Aug 21.
This study investigated the effects of extremely low frequency electromagnetic fields (ELF-EMFs) on human fetal scleral fibroblasts (HFSFs). HFSFs were subjected to 50 Hz artificial ELF-EMFs generated by Helmholtz coils with 0.1, 0.2, 0.5, and 1.0 mT field intensities for 6 to 48 h. The viability and factors involved in scleral structuring of HFSFs were determined. The growth rate of HFSFs significantly decreased after only 24 h of exposure to ELF-EMFs (0.2 mT). The messenger RNA (mRNA) expression of collagen type I (COL1A1) decreased and expression of matrix metalloproteinase-2 (MMP-2) increased significantly. There was a decrease in tissue inhibitor of MMP-2 mRNA levels between treated and control cells only at the 1.0 mT intensity level. Transforming growth factor beta-2 mRNA increased in exposed cells, and, simultaneously, fibroblast growth factor-2 mRNA levels decreased. The protein expressions of COL1A1 and MMP-2 were also significantly altered subsequent to exposure (p < 0.05). This study shows that ELF-EMFs had biological effects on HFSFs and could cause abnormality in scleral collagen.
本研究调查了极低频电磁场（ELF-EMFs）对人胎儿巩膜成纤维细胞（HFSFs）的影响。将HFSFs置于由亥姆霍兹线圈产生的50 Hz人工ELF-EMFs中，场强分别为0.1、0.2、0.5和1.0 mT，持续6至48小时。测定了HFSFs的活力以及参与巩膜结构形成的相关因子。暴露于ELF-EMFs（0.2 mT）仅24小时后，HFSFs的生长速率显著降低。I型胶原蛋白（COL1A1）的信使核糖核酸（mRNA）表达下降，而基质金属蛋白酶-2（MMP-2）的表达显著增加。仅在1.0 mT强度水平下，处理组细胞与对照组细胞相比，MMP-2的组织抑制剂mRNA水平有所下降。暴露细胞中转化生长因子β-2 mRNA增加，同时，成纤维细胞生长因子-2 mRNA水平下降。暴露后，COL1A1和MMP-2的蛋白表达也发生了显著改变（p < 0.05）。本研究表明，ELF-EMFs对HFSFs具有生物学效应，并可能导致巩膜胶原蛋白异常。
