Arnold R G, DiChristina T J, Hoffmann M R
Appl Environ Microbiol. 1986 Aug;52(2):281-9. doi: 10.1128/aem.52.2.281-289.1986.
Aerobic respiration and dissimilative iron reduction were studied in pure, batch cultures of Pseudomonas sp. strain 200 ("Pseudomonas ferrireductans"). Specific respiratory inhibitors were used to identify elements of electron transport chains involved in the reduction of molecular oxygen and Fe(III). When cells were grown at a high oxygen concentration, dissimilative iron reduction occurred via an abbreviated electron transport chain. The induction of alternative respiratory pathways resulted from growth at low oxygen tension (less than 0.01 atm [1 atm = 101.29 kPa]). Induced cells were capable of O2 utilization at moderately increased rates; dissimilative iron reduction was accelerated by a factor of 6 to 8. In cells grown at low oxygen tension, dissimilative iron reduction appeared to be uncoupled from oxidative phosphorylation. Models of induced and uninduced electron transport chains, including a mathematical treatment of chemical inhibition within the uninduced, aerobic electron transport system, are presented. In uninduced cells respiring anaerobically, electron transport was limited by ferrireductase activity. This limitation may disappear among induced cells.
在铜绿假单胞菌菌株200（“铁还原假单胞菌”）的纯分批培养物中研究了有氧呼吸和异化铁还原。使用特定的呼吸抑制剂来鉴定参与分子氧和Fe(III)还原的电子传递链元件。当细胞在高氧浓度下生长时，异化铁还原通过缩短的电子传递链发生。替代呼吸途径的诱导是由于在低氧张力（小于0.01 atm [1 atm = 101.29 kPa]）下生长所致。诱导细胞能够以适度增加的速率利用氧气；异化铁还原加速了6至8倍。在低氧张力下生长的细胞中，异化铁还原似乎与氧化磷酸化解偶联。本文提出了诱导和未诱导电子传递链的模型，包括对未诱导的有氧电子传递系统内化学抑制的数学处理。在厌氧呼吸的未诱导细胞中，电子传递受铁还原酶活性限制。这种限制在诱导细胞中可能消失。
