Obuekwe C O, Westlake D W, Cook F D
Can J Microbiol. 1981 Jul;27(7):692-7. doi: 10.1139/m81-107.
A Pseudomonas sp. isolated from crude oil reduced ferric ions (Fe(III)) to ferrous ions (Fe(II)). In the presence of nitrate (NO3-) after prolonged incubation, the amount of Fe(II) was lower than in its absence. However, during short incubation periods, the presence of NO3- significantly increased (99.5% confidence limit) the amount of Fe(II) produced. The decrease in Fe(II) on prolonged incubation was associated with increased production and accumulation of nitrite (NO2-). Under low NO3- levels, where the production of NO2- was limited, a decrease in NO2- concentration was accompanied by an increase in Fe(II) production to levels comparable with those obtained in the absence of NO3-. Preinduction of cells for nitrate reductase, which favoured rapid NO2- production, resulted in a more rapid decrease in Fe(II) production than in cells that were not preinduced. It is proposed that the inhibitory effect of NO3- on microbial reduction of Fe(III) is due to a secondary reaction, which involves the chemical oxidation of Fe(II) by NO2-.
从原油中分离出的一种假单胞菌属细菌可将铁离子（Fe(III)）还原为亚铁离子（Fe(II)）。在长时间培养且存在硝酸盐（NO₃⁻）的情况下，Fe(II)的量低于不存在硝酸盐时的量。然而，在短时间培养期间，NO₃⁻的存在显著增加了（99.5%置信限）Fe(II)的生成量。长时间培养后Fe(II)的减少与亚硝酸盐（NO₂⁻）生成和积累的增加有关。在低NO₃⁻水平下，NO₂⁻的生成受到限制，NO₂⁻浓度的降低伴随着Fe(II)生成量增加至与不存在NO₃⁻时相当的水平。对细胞进行硝酸还原酶预诱导有利于快速生成NO₂⁻，这导致Fe(II)生成量的下降比未预诱导的细胞更快。有人提出，NO₃⁻对微生物还原Fe(III)的抑制作用是由于一个次级反应，该反应涉及NO₂⁻对Fe(II)的化学氧化。
