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使用荧光增白剂快速诊断棘阿米巴角膜炎

Rapid diagnosis of Acanthamoeba keratitis using calcofluor white.

作者信息

Wilhelmus K R, Osato M S, Font R L, Robinson N M, Jones D B

出版信息

Arch Ophthalmol. 1986 Sep;104(9):1309-12. doi: 10.1001/archopht.1986.01050210063026.

DOI:10.1001/archopht.1986.01050210063026
PMID:2428343
Abstract

Calcofluor white (CFW) is a chemofluorescent dye with an affinity for the polysaccharide polymers of amebic cysts. Using CFW staining with fluorescent microscopy, we demonstrated amebic cysts in corneal scrapings and keratectomy specimens from four patients with culture-proved Acanthamoeba keratitis and from one in whom CFW was the only positive laboratory test. Calcofluor white staining is simple, rapid, and highly reliable in the diagnosis of Acanthamoeba keratitis.

摘要

荧光增白剂(CFW)是一种对阿米巴包囊的多糖聚合物具有亲和力的化学荧光染料。通过荧光显微镜下的CFW染色,我们在4例经培养证实为棘阿米巴角膜炎患者的角膜刮片和角膜切除术标本中,以及在1例CFW是唯一阳性实验室检查结果的患者中,发现了阿米巴包囊。荧光增白剂染色在棘阿米巴角膜炎的诊断中简单、快速且高度可靠。

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Rapid diagnosis of Acanthamoeba keratitis using calcofluor white.使用荧光增白剂快速诊断棘阿米巴角膜炎
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Cellulose binding and the timing of expression influence protein targeting to the double-layered cyst wall of .纤维素结合和表达时机影响蛋白质靶向到. 的双层囊壁。
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