Giannaki Maria, Kakourou Talia, Theodoridou Maria, Syriopoulou Vassiliki, Kabouris Marios, Louizou Eirini, Chrousos George
First Department of Pediatrics, Athens Medical School, Aghia Sophia Children's Hospital, Athens, Greece.
Pediatr Dermatol. 2013 Nov-Dec;30(6):730-5. doi: 10.1111/pde.12113. Epub 2013 Apr 4.
The human papillomavirus (HPV) infects the squamous epithelium of the skin and produces common warts, plantar warts, and flat warts, which occur commonly on the hands, face, and feet. The objective of this study was to determine the presence of HPV in warts in children in order to associate the virus with the disease. Sixty-eight children with clinically diagnosed cutaneous warts were recruited. Skin biopsy samples were examined and DNA was extracted using a commercially available kit. To distinguish between the HPV types, we used a specific pair of primers to amplify the HPV DNA. Polymerase chain reaction amplification of the L1 region was followed by restriction fragment length polymorphism analysis and Luminex xMAP technology. HPV 57 was the predominant type in our study, although the detection of the high-risk HPV type 16 in 33% of our positive samples indicates the presence of mucosal high-risk HPV types in the skin of children. It seems that the newly introduced Luminex assay maximized the discrimination of genotypes even in the case of multiple HPV infections. Or findings also suggest the presence of high-risk HPV types in cutaneous warts.
人乳头瘤病毒（HPV）感染皮肤的鳞状上皮，引发寻常疣、跖疣和扁平疣，这些疣常见于手部、面部和足部。本研究的目的是确定儿童疣中HPV的存在情况，以便将该病毒与疾病联系起来。招募了68名临床诊断为皮肤疣的儿童。对皮肤活检样本进行检查，并使用市售试剂盒提取DNA。为区分HPV类型，我们使用一对特异性引物扩增HPV DNA。对L1区域进行聚合酶链反应扩增后，进行限制性片段长度多态性分析和Luminex xMAP技术检测。HPV 57是我们研究中的主要类型，尽管在33%的阳性样本中检测到高危型HPV 16，这表明儿童皮肤中存在黏膜高危型HPV。新引入的Luminex检测方法似乎即使在多重HPV感染的情况下也能最大限度地实现基因型鉴别。我们的研究结果还表明皮肤疣中存在高危型HPV。
