Faculty of Medicine, Pathology, Microbiology, and Forensic Medicine, University of Jordan, Amman, Jordan.
J Med Virol. 2013 Jun;85(6):1058-62. doi: 10.1002/jmv.23519.
The epidemiology of human papillomaviruses (HPVs) genotype distribution of cutaneous warts in Jordanian patients were studied. A total of 200 samples were collected using skin swabs from patients with warts attending the dermatology clinic at the Jordan University Hospital over the period of June 2010 to October 2010. Another 100 control samples were taken from healthy Jordanian individuals with no current or previous history of warts. DNA extraction and sequencing was carried out using PCR with the FAP primer pair to detect HPV DNA, followed by multiple-type-specific (Multiplex) PCR combined with DNA sequencing. The prevalence of HPV among Jordanian patients tested with warts diagnosed clinically was 82% (157/192); of these 45% (87/192) were detected by FAP PCR method, and 37% (70/192) were detected by multiplex PCR method. Sequencing of the FAP positive samples shows that HPV 2 was associated with the highest prevalence (36%), followed by HPV 27 (28%) and HPV 57 (21%). A total of 6% of healthy persons were positive for HPV DNA. In conclusion, this study demonstrates that alpha HPV types (HPV 2, HPV 27, and HPV 57) are associated with the most prevalent cutaneous warts in Jordanian patients.
本研究旨在探讨约旦患者皮肤疣中人乳头瘤病毒（HPV）基因型分布的流行病学情况。2010 年 6 月至 10 月期间，我们使用皮肤拭子从约旦大学医院皮肤科诊所就诊的疣患者中采集了 200 份样本。此外，我们还从 100 名没有当前或既往疣病史的健康约旦个体中采集了 100 份对照样本。使用 FAP 引物对进行 PCR 提取和测序以检测 HPV DNA，然后进行多重特异性（Multiplex）PCR 结合 DNA 测序。经临床诊断为疣的约旦患者中 HPV 的检出率为 82%（157/192）；其中 45%（87/192）通过 FAP PCR 方法检出，37%（70/192）通过多重 PCR 方法检出。对 FAP 阳性样本进行测序显示，HPV 2 型的检出率最高（36%），其次是 HPV 27 型（28%）和 HPV 57 型（21%）。共有 6%的健康个体 HPV DNA 阳性。综上所述，本研究表明，α HPV 型（HPV 2、HPV 27 和 HPV 57）与约旦患者中最常见的皮肤疣相关。
