Department of Biomedical Sciences, Molecular and Cellular Biology Program, Corvallis, USA.
Oregon State University, Corvallis, Oregon 97331-4801, USA.
Proteome Sci. 2013 Nov 28;11(1):45. doi: 10.1186/1477-5956-11-45.
Pathogenic mycobacteria are important agents causing human disease. Mycobacterium avium subsp. hominissuis (M. avium) is a species of recalcitrant environmental pathogen. The bacterium forms robust biofilms that allow it to colonize and persist in austere environments, such as residential and commercial water systems. M. avium is also an opportunistic pathogen that is a significant source of mortality for immune-compromised individuals. Proteins exposed at the bacterial surface play a central role in mediating the relationship between the bacterium and its environment. The processes underlying both biofilm formation and pathogenesis are directly dependent on this essential subset of the bacterial proteome. Therefore, the characterization of the surface-exposed proteome is an important step towards an improved understanding of the mycobacterial biology and pathogenesis. Here we examined the complement of surface exposed proteins from Mycobacterium avium 104, a clinical isolate and reference strain of Mycobacterium avium subsp. hominissuis. To profile the surface-exposed proteins of viable M. avium 104, bacteria were covalently labeled with a membrane impermeable biotinylation reagent and labeled proteins were affinity purified via the biotin-streptavidin interaction. The results provide a helpful snapshot of the surface-exposed proteome of this frequently utilized reference strain of M. avium. A Cu-Zn SOD knockout mutant, MAV_2043, a surface identified protein, was evaluated regarding its role in the survival in both macrophages and neutrophils.
致病分枝杆菌是引起人类疾病的重要病原体。鸟分枝杆菌亚种同源亚种（M. avium）是一种顽固的环境病原体。该细菌形成坚固的生物膜，使其能够在住宅和商业供水系统等恶劣环境中定殖和持续存在。M. avium 也是一种机会性病原体，是免疫功能低下个体死亡的重要来源。暴露在细菌表面的蛋白质在介导细菌与其环境之间的关系方面起着核心作用。生物膜形成和发病机制的过程直接依赖于细菌蛋白质组的这一基本亚组。因此，对表面暴露蛋白质组的特征描述是深入了解分枝杆菌生物学和发病机制的重要步骤。在这里，我们研究了来自鸟分枝杆菌 104 的表面暴露蛋白的成分，这是一种临床分离株和鸟分枝杆菌亚种同源亚种的参考菌株。为了分析活的 M. avium 104 的表面暴露蛋白，用一种膜不可渗透的生物素化试剂共价标记细菌，并用生物素-链霉亲和素相互作用通过亲和纯化标记蛋白。该结果提供了该经常使用的 M. avium 参考菌株表面暴露蛋白质组的有用快照。对一种表面鉴定的蛋白质，Cu-Zn SOD 敲除突变体 MAV_2043，进行了评估，以确定其在巨噬细胞和中性粒细胞中的生存能力的作用。
