Mannella C A
Biochim Biophys Acta. 1986 Sep 25;861(1):67-73. doi: 10.1016/0005-2736(86)90372-x.
The effects of phospholipases C and D on the state of order of the channels in the outer membranes of Neurospora mitochondria have been investigated by negative-stain electron microscopy and optical diffraction. Unlike the situation with phospholipase A2, treatment of the isolated membranes with phospholipase C or D does not induce crystallization of the channels in the membrane plane. Furthermore, treatment of already-formed periodic arrays of outer membrane channels with either phospholipase C or D causes loss of long-range order in the arrays. The latter result suggests that zwitterionic phospholipids may play an important role in stabilizing the periodic arrays of the channel-forming protein in this membrane.
通过负染电子显微镜和光学衍射研究了磷脂酶C和D对粗糙脉孢菌线粒体外膜通道有序状态的影响。与磷脂酶A2的情况不同，用磷脂酶C或D处理分离的膜不会诱导膜平面内通道的结晶。此外，用磷脂酶C或D处理已经形成的外膜通道周期性阵列会导致阵列中长程有序性的丧失。后一结果表明两性离子磷脂可能在稳定该膜中通道形成蛋白的周期性阵列中起重要作用。
