Mannella C A, Ribeiro A J, Frank J
Biophys J. 1987 Feb;51(2):221-6. doi: 10.1016/S0006-3495(87)83327-1.
Computer-averaged electron microscopic images of negatively stained crystalline arrays of fungal mitochondrial outer-membrane channels in the presence and absence of cytochrome c were compared. Neither the apo- nor the holo- forms of cytochrome c significantly changed the stain distribution in the protein regions of the channel arrays. However, both forms of cytochrome c caused significant stain exclusion from the lipid domains in the arrays, suggesting binding of the polypeptides at these loci. The implications of binding of apocytochrome c to clusters of exposed phospholipids on the mitochondrial outer membrane are discussed with respect to the mechanism of uptake of this polypeptide by mitochondria.
比较了在存在和不存在细胞色素c的情况下，真菌线粒体外膜通道负染色晶体阵列的计算机平均电子显微镜图像。细胞色素c的脱辅基形式和全酶形式均未显著改变通道阵列蛋白质区域的染色分布。然而，两种形式的细胞色素c都导致阵列中脂质区域出现明显的染色排斥，表明多肽在这些位点结合。本文讨论了脱辅基细胞色素c与线粒体外膜上暴露的磷脂簇结合的意义，涉及该多肽被线粒体摄取的机制。
