Blount P, Elder J, Lipkin W I, Southern P J, Buchmeier M J, Oldstone M B
Brain Res. 1986 Sep 24;382(2):257-65. doi: 10.1016/0006-8993(86)91335-1.
Nucleic acid hybridization and protein blotting procedures have allowed the specific detection of both RNA and protein gene products in whole body sections of a selected host. These procedures permit efficient and reproducible screening of both endogenous and exogenous (viral) gene products, thus facilitating the study of normal differentiation, the localization of virus and the monitoring of viral diseases. Although success with RNA hybridization has thus far been limited to exogenous viral gene expression, the protein immunoblotting procedures have the sensitivity to detect endogenous protein products with high resolution. These combined procedures should prove useful for the study of protein expression in numerous developmental systems.
核酸杂交和蛋白质印迹法能够特异性检测选定宿主全身切片中的RNA和蛋白质基因产物。这些方法可对内源和外源（病毒）基因产物进行高效且可重复的筛选，从而有助于研究正常分化、病毒定位以及监测病毒性疾病。尽管迄今为止RNA杂交仅成功用于检测外源病毒基因表达，但蛋白质免疫印迹法具有高灵敏度，能够高分辨率地检测内源蛋白质产物。这些联合方法在众多发育系统的蛋白质表达研究中应会证明是有用的。
