Englert M E, Ferguson K M, Suarez C R, Oronsky A L, Kerwar S S
Cell Immunol. 1986 Sep;101(2):373-9. doi: 10.1016/0008-8749(86)90150-4.
Using a combination of affinity chromatography procedures, anticollagen IgG was fractionated into three distinct populations. One population reacted only to conformational determinants, another population reacted only to structural determinants, and the third population reacted to both conformation and structural determinants. When these populations were tested for their arthritogenicity, only those fractions that reacted to conformational and to conformational and structural determinants were active in inducing clinical arthritis. Immunofluorescence analysis of the hind paw of recipient rats indicated that antibodies directed only to conformational and to both conformational and structural determinants bound to articular cartilage and activated the complement system. Antibodies directed strictly to structural determinants did not bind to articular cartilage and were nonarthritogenic.
通过结合亲和层析程序，抗胶原蛋白IgG被分离成三个不同的群体。一个群体仅对构象决定簇有反应，另一个群体仅对结构决定簇有反应，第三个群体对构象和结构决定簇都有反应。当测试这些群体的致关节炎性时，只有那些对构象以及对构象和结构决定簇都有反应的部分在诱导临床关节炎方面具有活性。对受体大鼠后爪的免疫荧光分析表明，仅针对构象以及针对构象和结构决定簇的抗体与关节软骨结合并激活补体系统。严格针对结构决定簇的抗体不与关节软骨结合且无致关节炎性。
