Marchitto K S, Kindt T J, Norgard M V
Cell Immunol. 1986 Sep;101(2):633-42. doi: 10.1016/0008-8749(86)90173-5.
Evidence is presented for the association of class I major histocompatibility complex (MHC) antigens with the surface of Treponema pallidum during infection. A monoclonal antibody (IgG2a) directed against a murine H-2Kb epitope of public specificity reacted with the cell surface of T. pallidum, as assayed by the binding of protein A-colloidal gold in immunoelectron microscopy. Monoclonal antibodies directed against class I rabbit MHC antigens also reacted in immunofluorescence assays with material on the surface of rabbit-cultivated T. pallidum. In addition, impression smears of human syphilitic genital ulcers that were darkfield-positive for the presence of spirochetes were tested in immunofluorescence assays with monoclonal antibodies directed against human MHC antigens; antibody directed against HLA-ABC (class I) was reactive whereas antibody directed against HLA-DR (class II) was nonreactive. Results of the study suggest that the association of host-derived class I MHC antigens or molecular mimicry may play a role in T. pallidum evasion of host immune defenses.
有证据表明，在感染期间，I类主要组织相容性复合体（MHC）抗原与梅毒螺旋体表面有关联。一种针对具有公共特异性的小鼠H-2Kb表位的单克隆抗体（IgG2a）与梅毒螺旋体的细胞表面发生反应，这是通过免疫电子显微镜中蛋白A-胶体金的结合来测定的。针对I类兔MHC抗原的单克隆抗体在免疫荧光试验中也与兔培养的梅毒螺旋体表面的物质发生反应。此外，对暗视野检查发现有螺旋体的人类梅毒生殖器溃疡的印片进行了免疫荧光试验，用针对人类MHC抗原的单克隆抗体进行检测；针对HLA-ABC（I类）的抗体有反应，而针对HLA-DR（II类）的抗体无反应。该研究结果表明，宿主来源的I类MHC抗原的关联或分子模拟可能在梅毒螺旋体逃避宿主免疫防御中起作用。
