Marchitto K S, Selland-Grossling C K, Norgard M V
Infect Immun. 1986 Jan;51(1):168-76. doi: 10.1128/iai.51.1.168-176.1986.
Radioimmunoprecipitation (RIP) and Western blot analyses with specific anti-Treponema pallidum subsp. pallidum monoclonal antibodies were used to identify antigens with apparent masses of 102, 84, 54, 53, 52, 47, 32, 29, and 24 kilodaltons (kDa). Cross-reactivity of these antibodies with T. pallidum subsp. pertenue antigens and lack of cross-reactivity with T. phagedenis biotype Reiter, T. vincentii, T. refringens, T. scoliodontum, and T. denticola were also demonstrated by RIP and Western blot analyses. Reactivities in the T. pallidum immobilization test, along with the RIP of lactoperoxidase-catalyzed iodination products, suggested that the identified antigens were surface associated. The abundance and surface association of the 47- and 84-kDa antigens were supported by reactivity in the microhemagglutination test for T. pallidum and by strong reactivity of monoclonal antibodies upon indirect immunofluorescence assays with rabbit-cultivated T. pallidum subsp. pallidum, respectively, but not with T. phagedenis biotype Reiter. Anti-47-kDa and anti-84-kDa monoclonal antibodies were also reactive in indirect immunofluorescence assays using treponemes found in dark-field-positive smears of human genital ulcers.
采用放射免疫沉淀（RIP）和蛋白质免疫印迹分析，使用特异性抗梅毒螺旋体苍白亚种单克隆抗体来鉴定表观质量为102、84、54、53、52、47、32、29和24千道尔顿（kDa）的抗原。通过RIP和蛋白质免疫印迹分析还证明了这些抗体与梅毒螺旋体 pertenue抗原的交叉反应性，以及与溃蚀性螺旋体生物型赖特氏菌、奋森氏螺旋体、解脲脲原体、曲齿螺旋体和齿垢密螺旋体缺乏交叉反应性。梅毒螺旋体固定试验中的反应性，以及乳过氧化物酶催化碘化产物的RIP，表明所鉴定的抗原与表面相关。梅毒螺旋体微量血凝试验中的反应性以及单克隆抗体在用兔培养的梅毒螺旋体苍白亚种进行间接免疫荧光试验时的强反应性，分别支持了47-kDa和84-kDa抗原的丰度和表面相关性，但与溃蚀性螺旋体生物型赖特氏菌无反应。抗47-kDa和抗84-kDa单克隆抗体在使用人生殖器溃疡暗视野阳性涂片发现的螺旋体进行的间接免疫荧光试验中也有反应。
