非洲爪蟾卵母细胞中B细胞刺激因子-1的体外翻译

In vitro translation of B-cell stimulatory factor-1 in Xenopus laevis oocytes.

作者信息

Brown M A, Watson C, Ohara J, Paul W E

出版信息

Cell Immunol. 1986 Apr 1;98(2):538-43. doi: 10.1016/0008-8749(86)90313-8.

Abstract

B-cell stimulatory factor-1 (BSF-1) can be translated in vitro in Xenopus laevis oocytes. This activity is blocked by an antibody to BSF-1. The RNA species coding for BSF-1 activity sediments of approximately 8-9S and is separable from RNA coding for interleukin-2 activity which sediments at approximately 11.5S. Finally, the fact that BSF-1 can be translated in vitro confirms that functions attributed to BSF-1 do not depend on contamination with other biologically active molecules such as phorbol myristate acetate.

摘要

B细胞刺激因子-1（BSF-1）可在非洲爪蟾卵母细胞中进行体外翻译。该活性被抗BSF-1抗体所阻断。编码BSF-1活性的RNA种类沉降系数约为8 - 9S，且可与编码沉降系数约为11.5S的白细胞介素-2活性的RNA分离。最后，BSF-1可在体外翻译这一事实证实，归因于BSF-1的功能并不依赖于与其他生物活性分子如佛波醇肉豆蔻酸酯的污染。