Raffaello Tommaso, Chen Hongxin, Kohler Annegret, Asiegbu Fred O
University of Helsinki, Department of Forest Sciences, Latokartanonkaari 7, 00014, Helsinki, Finland; Viikki Doctoral Programme in Molecular Biosciences (VGSB), Viikinkaari 9, 00014, Helsinki, Finland.
Environ Microbiol. 2014 Jun;16(6):1654-67. doi: 10.1111/1462-2920.12321. Epub 2013 Nov 28.
The success of many wood decaying fungi lies in their ability to overcome unfavourable environmental conditions within and outside of litter and wood debris. Although so much has been learned about the ecology, taxonomy and physiology of several wood decaying basidiomycete fungi, the molecular basis for their survival in a diverse range of substrates and ecological habitats has been very little studied. Using the wood decay fungus (Heterobasidion annosum s.s.) as a model, we investigated its transcriptomic response when exposed to several environmental stressors (high and low temperature, osmotic stress, oxidative stress and nutrient starvation) and during growth on specific pine wood compartments (bark, sapwood and heartwood). Among other genes and pathways, we documented the specific induction of the major facilitator superfamily 1 and cytochrome P450 families at low temperature, and protein kinases together with transcription factors during starvation. On the other hand, during saprotrophic growth, we observed the induction of many glycosyl hydrolases, three multi-copper oxidases (MCO), five manganese peroxidases (MnP) and one oxidoreductase which are specific for wood degradation. This is the first study providing insights on the potential mechanisms for adaptation to abiotic stresses and pine heartwood degradation in H. annosum s.s.
许多木材腐朽真菌的成功之处在于它们有能力克服枯枝落叶和木材碎片内外不利的环境条件。尽管我们已经对几种木材腐朽担子菌的生态学、分类学和生理学有了很多了解，但对它们在各种底物和生态栖息地中生存的分子基础却研究甚少。以木材腐朽真菌（狭义异担子菌）为模型，我们研究了其在暴露于几种环境应激源（高温和低温、渗透应激、氧化应激和营养饥饿）时以及在特定松木组分（树皮、边材和心材）上生长期间的转录组反应。在其他基因和途径中，我们记录了低温下主要促进剂超家族1和细胞色素P450家族的特异性诱导，以及饥饿期间蛋白激酶和转录因子的诱导。另一方面，在腐生生长过程中，我们观察到许多糖基水解酶、三种多铜氧化酶（MCO）、五种锰过氧化物酶（MnP）和一种对木材降解具有特异性的氧化还原酶的诱导。这是第一项提供关于狭义异担子菌适应非生物胁迫和松木心材降解潜在机制见解的研究。
