Heard S R, Rasburn B, Matthews R C, Tabaqchali S
J Clin Microbiol. 1986 Sep;24(3):384-7. doi: 10.1128/jcm.24.3.384-387.1986.
The epidemiology of Clostridium difficile-associated disease is being elucidated with the development of typing schemes for the organism. We recently described a new typing scheme based on the incorporation of [35S]methionine into bacterial proteins followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Nine standard strains were identified. We report here some observations on the antigenic differences among these nine strains when studied by immunoblotting. Type-specific rabbit antiserum was raised against each of the nine standard strains. Immunoblotting of the strains with these antisera demonstrated, in addition to the presence of shared, common proteins, a type-specific response with homologous antisera. When [35S]methionine-labeled C. difficile proteins were immunoblotted with homologous and heterologous antisera, both the immunoblots and the autoradiographs demonstrated the same strain-specific response. These strain-specific proteins, which have been so useful for epidemiological and typing purposes, were also immunogenic.
随着针对艰难梭菌的分型方案的发展，艰难梭菌相关性疾病的流行病学正在得到阐明。我们最近描述了一种新的分型方案，该方案基于将[35S]甲硫氨酸掺入细菌蛋白质中，随后进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影。鉴定出了9个标准菌株。我们在此报告通过免疫印迹研究这9个菌株之间抗原差异的一些观察结果。针对这9个标准菌株中的每一个制备了型特异性兔抗血清。用这些抗血清对菌株进行免疫印迹显示，除了存在共同的、共享的蛋白质外，与同源抗血清存在型特异性反应。当用同源和异源抗血清对[35S]甲硫氨酸标记的艰难梭菌蛋白质进行免疫印迹时，免疫印迹和放射自显影片都显示出相同的菌株特异性反应。这些对流行病学和分型目的非常有用的菌株特异性蛋白质也是具有免疫原性的。
