针对艰难梭菌16S - 23S rRNA基因间隔区的聚合酶链反应（PCR）以及由116种不同PCR核糖体分型组成的文库构建。
PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes.
作者信息
Stubbs S L, Brazier J S, O'Neill G L, Duerden B I
机构信息
Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Cardiff, United Kingdom.
出版信息
J Clin Microbiol. 1999 Feb;37(2):461-3. doi: 10.1128/JCM.37.2.461-463.1999.
Abstract
A reference library of types of Clostridium difficile has been constructed by PCR ribotyping isolates (n = 2,030) from environmental (n = 89), hospital (n = 1,386), community practitioner (n = 395), veterinary (n = 27), and reference (n = 133) sources. The library consists of 116 distinct types identified on the basis of differences in profiles generated with PCR primers designed to amplify the 16S-23S rRNA gene intergenic spacer region. Isolates from 55% of infections in hospitals in the United Kingdom belonged to one ribotype (type 1), but this type was responsible for only 7. 5% of community infections.
摘要
通过对来自环境（89株）、医院（1386株）、社区医疗机构（395株）、兽医机构（27株）和参考菌株（133株）的艰难梭菌分离株（共2030株）进行PCR核糖体分型，构建了艰难梭菌类型参考文库。该文库包含116种不同类型，这些类型是根据用于扩增16S - 23S rRNA基因间隔区的PCR引物所产生的图谱差异来确定的。在英国医院55%的感染病例中分离出的菌株属于一种核糖体类型（1型），但该类型仅导致7.5%的社区感染。
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