Logvinenko N S, Solenov E I, Ivanova L N
Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2013 Sep;155(5):615-7. doi: 10.1007/s10517-013-2208-z.
The mechanism of aldosterone effects (10 nM) on the volume of the principal cells of cortical collecting ducts after hypoosmotic stress (280/140 mOsm/kg) was studied using fluorescence microscopy. The experiments with intracellular fluorescent dye calcein showed that aldosterone significantly decreases the amplitude and rate of increase in principal cell volume during hypoosmotic stress. Epithelial sodium channel blocker amiloride (10(-5)M) significantly attenuated the effects of aldosterone on the amplitude and rate of changes in cell volume. The obtained data attest to the contribution of epithelial sodium channel to the realization of rapid non-genomic effects of aldosterone on the amplitude and rate of changes in volume of the principal cells of cortical collecting ducts in rat kidney after hypoosmotic stress.
利用荧光显微镜研究了低渗应激（280/140 mOsm/kg）后醛固酮（10 nM）对皮质集合管主细胞体积的作用机制。用细胞内荧光染料钙黄绿素进行的实验表明，醛固酮显著降低了低渗应激期间主细胞体积增加的幅度和速率。上皮钠通道阻滞剂氨氯地平（10⁻⁵M）显著减弱了醛固酮对细胞体积变化幅度和速率的影响。所得数据证明，上皮钠通道有助于醛固酮在大鼠肾脏低渗应激后对皮质集合管主细胞体积变化幅度和速率产生快速非基因组效应。
