醛固酮对大鼠肾脏生后发育过程中集合管主细胞体积的快速非基因组效应及上皮钠通道的遗传异质性

Fast Nongenomic Effect of Aldosterone on the Volume of Principal Cells in Collecting Tube and Genetic Heterogeneity of Epithelial Sodium Channel in the Postnatal Ontogenesis of Rat Kidney.

作者信息

Logvinenko N S, Gerbek Yu E, Solenov E I, Ivanova L N

机构信息

Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.

出版信息

Bull Exp Biol Med. 2016 Mar;160(5):691-4. doi: 10.1007/s10517-016-3251-3. Epub 2016 Mar 29.

DOI:10.1007/s10517-016-3251-3

PMID:27021081

Abstract

The effects of amiloride, epithelial sodium pump inhibitor, on the fast nongenomic effect of aldosterone in principal cells of an isolated segment of the distal portion of renal collecting tubes were studied in 10-day-old and adult rats. Fluorescent staining with Calcein AM showed various effects of amiloride (10(-5) M) on the stabilizing effect of aldosterone (10 nM) in hypotonic shock (280/140 mOsm/kg). Amiloride attenuated by 30% the effect of aldosterone on the amplitude of principal cell swelling in adult animals and almost completely abolished this effect in 10-day rats (p<0.05). These age-specific differences in the contribution of the distal portion of the collecting tube to the nongenomic effect of aldosterone did not depend on genetic heterogeneity of its α-subunit.

摘要

在10日龄和成年大鼠中，研究了上皮钠泵抑制剂氨氯地平对肾集合管远端孤立节段主细胞中醛固酮快速非基因组效应的影响。用Calcein AM进行荧光染色显示，氨氯地平（10⁻⁵ M）对醛固酮（10 nM）在低渗休克（280/140 mOsm/kg）中的稳定作用有多种影响。在成年动物中，氨氯地平使醛固酮对主细胞肿胀幅度的作用减弱了30%，而在10日龄大鼠中几乎完全消除了这种作用（p<0.05）。集合管远端对醛固酮非基因组效应贡献的这些年龄特异性差异并不取决于其α亚基的遗传异质性。