Mariani M, Nucci D, Bracci L, Neri P, Antoni G
J Immunol Methods. 1986 Sep 27;92(2):189-93. doi: 10.1016/0022-1759(86)90165-1.
A rapid and sensitive method is described for the determination of the antigen-specific immunoglobulin contents of ascitic fluids and/or antisera. Immunoglobulins are quantitatively separated from the biological fluid by immunoaffinity chromatography on the insolubilized antigen using the Millipore Millititer filtration system to perform the simultaneous assay of multiple samples. The specific immunoglobulins are then eluted at acidic pH and their concentration is estimated by solid-phase competitive radioimmunoassay. Radioimmunoassay values, corrected for dilutions, give the antigen-specific immunoglobulin concentration of ascitic fluids and/or antisera.
本文描述了一种快速且灵敏的方法，用于测定腹水和/或抗血清中抗原特异性免疫球蛋白的含量。使用密理博微量滴定过滤系统，通过在固定化抗原上进行免疫亲和层析，从生物流体中定量分离免疫球蛋白，以同时检测多个样品。然后在酸性pH条件下洗脱特异性免疫球蛋白，并通过固相竞争放射免疫测定法估算其浓度。经稀释校正后的放射免疫测定值，即为腹水和/或抗血清中抗原特异性免疫球蛋白的浓度。
