Kim S, Tuck M, Ho L L, Campagnoni A T, Barbarese E, Knobler R L, Lublin F D, Chanderkar L P, Paik W K
J Neurosci Res. 1986;16(2):357-65. doi: 10.1002/jnr.490160203.
Myelin basic protein (MBP)-specific protein-arginine N-methyltransferase (protein methylase I) activity in homozygous shiverer (shi/shi) mutant mouse brain is significantly higher than in the normal littermate brain at the onset of myelination. While the enzyme activity (expressed as pmol of S-adenosyl-L-[methyl-14C]methionine used/min/mg enzyme protein) increases coincidently during the period of myelination in the normal brain (15-18 days of age), it decreases significantly in the mutant brain during this period of time. These results are in contrast to those found with another dysmyelinating mutant, jimpy (jp/Y) mice, in which the enzyme activity in the mutant brains is similar to that in the normal animals but remains unchanged during the myelination process. There is no difference in the weight and protein concentration of the normal and shiverer mutant brains with corresponding ages, and the histone-specific protein methylase I activity is also unaffected in the shiverer brain.
在髓鞘形成开始时，纯合子颤抖（shi/shi）突变小鼠脑中的髓鞘碱性蛋白（MBP）特异性蛋白精氨酸N-甲基转移酶（蛋白甲基化酶I）活性显著高于正常同窝小鼠脑。在正常脑（15 - 18日龄）的髓鞘形成期，该酶活性（以每分钟每毫克酶蛋白使用的S-腺苷-L-[甲基-14C]甲硫氨酸的皮摩尔数表示）同时增加，而在此期间突变脑内该酶活性则显著下降。这些结果与另一种脱髓鞘突变体——jimpy（jp/Y）小鼠的结果相反，在jimpy小鼠中，突变脑内的酶活性与正常动物相似，且在髓鞘形成过程中保持不变。正常和颤抖突变小鼠脑在相应年龄时的重量和蛋白质浓度没有差异，且颤抖脑内组蛋白特异性蛋白甲基化酶I活性也未受影响。
