Chanderkar L P, Shanker G, Knobler R L, Lublin F D, Paik W K, Kim S
Neurochem Res. 1987 May;12(5):445-9. doi: 10.1007/BF00972296.
Mice with the dysmyelinating mutation shiverer were studied by measuring the activity of two protein methylases and myelin marker enzymes in the brain. It was observed that S-adenosylmethionine:protein-lysine N-methyltransferase (protein methylase III, EC. 2.1.1.43) activity is significantly reduced in phenotypically affected homozygous shiverer (shi/shi) mutant mouse brain compared to the unaffected heterozygous littermate brain. This reduction in enzyme activity is manifested mainly by reduced formation of trimethyllysine during the in vitro methylation of histone. In contrast, myelin marker enzymes such as 2',3'-cyclic nucleotide 3'-phosphohydrolase and 5'-nucleotidase as well as S-adenosyl-methionine:protein-carboxyl O-methyltransferase (protein methylase II, EC. 2.1.1.24) activities were not significantly affected in these strains of mice.
通过测量大脑中两种蛋白质甲基化酶和髓鞘标记酶的活性，对患有脱髓鞘突变的颤抖小鼠进行了研究。观察到，与未受影响的杂合子同窝小鼠大脑相比，在表型受影响的纯合颤抖（shi/shi）突变小鼠大脑中，S-腺苷甲硫氨酸：蛋白质赖氨酸N-甲基转移酶（蛋白质甲基化酶III，EC. 2.1.1.43）的活性显著降低。酶活性的这种降低主要表现为组蛋白体外甲基化过程中三甲基赖氨酸形成的减少。相比之下，髓鞘标记酶如2',3'-环核苷酸3'-磷酸水解酶和5'-核苷酸酶以及S-腺苷甲硫氨酸：蛋白质羧基O-甲基转移酶（蛋白质甲基化酶II，EC. 2.1.1.24）的活性在这些品系的小鼠中未受到显著影响。
