Department of Biology, University of Naples- "Federico II", Naples, Italy.
Nutrition. 2014 Jan;30(1):75-81. doi: 10.1016/j.nut.2013.05.022.
We assessed the alterations in mitochondrial function in skeletal muscle that were elicited by short-term high-fat feeding in sedentary rats.
Two groups of rats were pair-fed for 1 wk and received a low-fat or high-fat diet. Body composition, energy balance, and glucose homeostasis were measured. Mitochondrial mass, oxidative capacity, and energetic efficiency as well as parameters of oxidative stress and antioxidant defense were evaluated in subsarcolemmal and intermyofibrillar mitochondria from the skeletal muscle.
Body energy, lipid content, and metabolic efficiency were significantly higher and energy expenditure was significantly decreased among rats that were fed a high-fat diet, as compared with controls. Skeletal muscle mitochondrial energetic efficiency, oxidative capacity for lipid substrates, and antioxidant defense were significantly increased in rats that were fed a high-fat diet as compared with controls.
Acute isocaloric high-fat feeding is able to induce increased phosphorylation efficiency in skeletal muscle subsarcolemmal and intermyofibrillar mitochondria. This modification implies a reduced oxidation of energy substrates that may contribute to the early onset of obesity.
我们评估了短期高脂喂养对久坐大鼠骨骼肌中线粒体功能的改变。
两组大鼠进行了为期 1 周的配对喂养，分别给予低脂或高脂饮食。测量了身体成分、能量平衡和葡萄糖稳态。评估了骨骼肌亚肌小节和肌间线粒体的线粒体质量、氧化能力和能量效率以及氧化应激和抗氧化防御的参数。
与对照组相比，高脂饮食喂养的大鼠体能量、脂质含量和代谢效率显著升高，而能量消耗显著降低。与对照组相比，高脂饮食喂养的大鼠骨骼肌线粒体能量效率、脂质底物的氧化能力和抗氧化防御显著增加。
急性等热量高脂喂养能够诱导骨骼肌亚肌小节和肌间线粒体磷酸化效率增加。这种修饰意味着能量底物的氧化减少，这可能有助于肥胖的早期发生。
