Contreras T J, Hunt S M, Lionetti F J, Valeri C R
Transfusion. 1978 Jan-Feb;18(1):46-53. doi: 10.1046/j.1537-2995.1978.18178118565.x.
Human granulocytes were isolated from blood either by counterflow centrifugation in a Beckman JE-6 rotor or by sedimentation of the red blood cells with dextran and centrifugation of the granulocyte-rich plasma. The stability of the granulocytes was assessed during storage in the liquid state by measurements of granulocyte loss, volume distribution characteristics, and ability to produce fluorescein in their cytoplasm and to exclude ethidium bromide from their nuclei. After storage at 4 C for 2 days in phosphate-buffered saline with a pH of 7.1 containing 5 g/dl human albumin + 0.46 g/dl dextrose or 1 g/dl Physiogel + 0.46 g/dl dextrose, the granulocytes were adequately preserved from the in vitro measurements.
人粒细胞可通过在贝克曼JE - 6转子中进行逆流离心，或通过用葡聚糖沉降红细胞并离心富含粒细胞的血浆，从血液中分离出来。通过测量粒细胞损失、体积分布特征以及在其细胞质中产生荧光素和将溴化乙锭排除在细胞核外的能力，评估粒细胞在液态储存期间的稳定性。在含有5 g/dl人白蛋白+ 0.46 g/dl葡萄糖或1 g/dl Physiogel + 0.46 g/dl葡萄糖、pH为7.1的磷酸盐缓冲盐水中于4℃储存2天后，从体外测量结果来看，粒细胞得到了充分保存。
