Kurtz S R, Carciero R, Valeri C R
Transfusion. 1979 Jul-Aug;19(4):398-403. doi: 10.1046/j.1537-2995.1979.19479250176.x.
The uptake of 51chromium by human granulocytes isolated from blood by counterflow centrifugation is influenced by the composition of the suspension medium, the incubation period, the temperature and the amount of 51chromium used. Counterflow centrifugation-elutriation isolated approximately 98 per cent of the granulocytes from the blood. Acid-citrate-dextrose (ACD) plasma, citrate-phosphate-dextrose (CPD) plasma and heparinized plasma as resuspension media produced similar labeling results, whereas phosphate-buffered saline resulted in three times greater uptake in 51chromium. The greatest uptake of 51chromium (4%) was achieved after incubation of 37 C for one hour in autologous plasma, and granulocyte integrity and metabolic function in vitro were not adversely affected.
通过逆流离心从血液中分离出的人粒细胞对51铬的摄取受悬浮培养基的组成、孵育时间、温度以及所用51铬量的影响。逆流离心淘析从血液中分离出了约98%的粒细胞。作为重悬培养基的酸性枸橼酸盐葡萄糖（ACD）血浆、枸橼酸盐磷酸盐葡萄糖（CPD）血浆和肝素化血浆产生了相似的标记结果，而磷酸盐缓冲盐水导致51铬的摄取量增加了两倍。在自体血浆中于37℃孵育1小时后实现了51铬的最大摄取量（4%），且粒细胞的完整性和体外代谢功能未受到不利影响。
