State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Department of Food and Environment Engineering, East University of Heilongjiang, Harbin 150086, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.
Bioresour Technol. 2014;152:124-9. doi: 10.1016/j.biortech.2013.11.011. Epub 2013 Nov 14.
This study aimed at exploring the potential of volatile fatty acids (VFAs) produced from excess sludge (ES) as a new cost-effective internal carbon source in wastewater treatment processes. The optimal condition for VFA accumulation and the bacterial community structure in the bio-production of VFAs from ES were investigated at different pH conditions. Denaturing gradient gel electrophoresis (DGGE) was performed to characterize the bacterial community structure of ES. The results showed that the optimal pH was 10.0 for VFA production, and acetic and propionic acids were the dominant acid species. Pseudomonas sp. was identified as the major bacteria capable of utilizing organic carbon at all pHs. Uncultured bacterium (AB658278) adapted well at high pH. Uncultured bacteria (KC633537 and JN596370) were mainly responsible for anaerobic degradation of ES. The study demonstrated the variation of bacterial community structures at pHs and the close correlation with the VFA accumulation.
本研究旨在探索从剩余污泥（ES）中产生的挥发性脂肪酸（VFAs）作为废水处理过程中一种新型经济有效的内部碳源的潜力。在不同 pH 条件下，研究了 ES 中 VFA 生物生产的最佳条件和细菌群落结构。采用变性梯度凝胶电泳（DGGE）对 ES 细菌群落结构进行了表征。结果表明，产 VFA 的最佳 pH 值为 10.0，且以乙酸和丙酸为主。假单胞菌被鉴定为所有 pH 值下利用有机碳的主要细菌。在高 pH 值下，未培养细菌（AB658278）适应良好。未培养细菌（KC633537 和 JN596370）主要负责 ES 的厌氧降解。该研究表明了 pH 值变化时细菌群落结构的变化，并与 VFA 积累密切相关。
