School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
Bioresour Technol. 2014 Feb;153:87-94. doi: 10.1016/j.biortech.2013.11.049. Epub 2013 Nov 27.
Batch fermentations of waste activated sludge (WAS) at alkaline pH with different inocula were performed. Paper mill anaerobic granular sludge (PAS) and dyeing mill anaerobic sludge (DAS) were used as inocula. At pH 10 the inoculation did not increase the volatile fatty acids (VFAs) production compared to the non-inoculated samples fermented in the same conditions, and the maximal VFAs yield of non-inoculated WAS was higher than inoculated WAS. However, at pH 9 the inoculation with PAS increased the sludge hydrolysis and VFAs production was 1.7-fold higher than that in non-inoculated WAS (yield 52.40mg/g of volatile solid). Denaturing gradient gel electrophoresis analysis revealed that 3 bacterial species, identified as Proteocatella, Tepidibacter, and Clostridium, disappeared when inoculated with PAS at pH 9 or at pH⩾10. The results showed that the inoculation with PAS can be helpful to achieve a relatively high VFAs production from WAS in a moderate alkaline environment.
采用不同接种物在碱性 pH 值下进行了废活性污泥（WAS）的分批发酵。造纸厂厌氧颗粒污泥（PAS）和染整厂厌氧污泥（DAS）被用作接种物。在 pH 值为 10 时，与在相同条件下发酵的未接种样品相比，接种并没有增加挥发性脂肪酸（VFAs）的产生，并且未接种 WAS 的最大 VFAs 产率高于接种 WAS。然而，在 pH 值为 9 时，接种 PAS 增加了污泥水解，VFAs 的产生比未接种 WAS 高出 1.7 倍（挥发性固体的产率为 52.40mg/g）。变性梯度凝胶电泳分析表明，当在 pH 值为 9 或 pH 值⩾10 时用 PAS 接种时，有 3 种细菌，鉴定为Proteocatella、Tepidibacter 和 Clostridium，消失了。结果表明，在适度碱性环境中，接种 PAS 有助于从 WAS 中获得相对较高的 VFAs 产量。
