School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China; Shanghai Institute of Pharmaceutical Industry, 1111 Zhongshan Beiyi Road, Shanghai 200437, PR China.
School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.
J Control Release. 2014 Jan 28;174:126-36. doi: 10.1016/j.jconrel.2013.11.020. Epub 2013 Dec 1.
The plasma membrane as a selectively permeable barrier of living cells is essential to cell survival and function. In many cases, however, the efficient passage of exogenous bioactive molecules through the plasma membrane remains a major hurdle for intracellular delivery of cargoes. During the last two decades, the potential of peptides for drug delivery into cells has been highlighted by the discovery of numerous cell-penetrating peptides (CPPs). CPPs serving as carriers can successfully intracellular transport cargoes such as siRNA, nucleic acids, proteins, small molecule therapeutic agents, quantum dots and MRI contrast agents. This review mainly introduces recent advances of CPPs as new carriers for the development of cellular imaging, nuclear localization, pH-sensitive and thermally targeted delivery systems. In particular, we highlight the exploiting of the synergistic effects of targeting ligands and CPPs. What's more, the classification and cellular uptake mechanisms of CPPs are briefly discussed as well.
细胞膜作为活细胞的选择性渗透屏障，对于细胞的存活和功能至关重要。然而，在许多情况下，外源生物活性分子有效地穿过细胞膜仍然是细胞内货物输送的主要障碍。在过去的二十年中，通过发现许多穿透肽（CPPs），肽类在药物递送到细胞中的潜力得到了强调。作为载体的 CPP 可以成功地将货物如 siRNA、核酸、蛋白质、小分子治疗剂、量子点和 MRI 造影剂等输送到细胞内。这篇综述主要介绍了 CPP 作为细胞成像、核定位、pH 敏感和热靶向递药系统新载体的最新进展。特别地，我们强调了靶向配体和 CPP 协同作用的利用。此外，CPP 的分类和细胞摄取机制也进行了简要讨论。
