do Nascimento Glauce Crivelaro, Leite-Panissi Christie Ramos Andrade
Psychobiology Graduate Program, School of Philosophy, Science and Literature of Ribeirão Preto, University of São Paulo, Ribeirão Preto, 14040-901 SP, Brazil.
Psychobiology Graduate Program, School of Philosophy, Science and Literature of Ribeirão Preto, University of São Paulo, Ribeirão Preto, 14040-901 SP, Brazil; Department of Morphology, Physiology and Basic Pathology, Ribeirão Preto Dentistry School, University of São Paulo, Ribeirão Preto, 14040-904 SP, Brazil.
Physiol Behav. 2014 Feb 10;125:1-7. doi: 10.1016/j.physbeh.2013.11.009. Epub 2013 Nov 27.
Temporomandibular disorder (TMD) is prevalent in dental clinics and can involve problems with the masticatory muscles or the temporomandibular joints (TMJ). The pain of TMD is frequently associated with inflammation in the TMJs, but it's etiology is considered to be multifactorial and includes biologic, behavioral, environmental, social, emotional and cognitive factors. The purpose of this investigation was to evaluate the anxiety-like behavior in rats exposed to temporomandibular inflammation via injection of Freund's Adjuvant (CFA) with the elevated plus maze (EPM) and light/dark box (LDB) tests and to evaluate nociceptive behavior with the von Frey test at different periods. Moreover, this study measured TMJ inflammation using plasma extravasation (Evans blue test) and the intraarticular infiltration of polymorphonuclear neutrophils (myeloperoxidase quantification). The results showed that rats that were submitted to TMJ inflammation exhibited a decreased number of entries into the open arms of the EPM and a decrease in the time spent in the light compartment and in the number of transitions in the LDB. Additionally, the number of entries in closed arms in the EPM, used as indicator of locomotor activity, did not alter between treatments. Furthermore, increases in mechanical sensitivity and increases in plasma extravasation in the joint tissue occurred throughout the inflammation process, along with an increase in myeloperoxidase in the synovial fluid of TMJ. Our results suggest that the temporomandibular inflammation induced by CFA produced anxiety-like behaviors in rats and induced nociceptive behavior across different periods of inflammation.
颞下颌关节紊乱病（TMD）在牙科诊所中很常见，可能涉及咀嚼肌或颞下颌关节（TMJ）的问题。TMD的疼痛通常与TMJ的炎症有关，但其病因被认为是多因素的，包括生物、行为、环境、社会、情感和认知因素。本研究的目的是通过高架十字迷宫（EPM）和明暗箱（LDB）试验评估经弗氏完全佐剂（CFA）注射诱发颞下颌关节炎症的大鼠的焦虑样行为，并在不同时期用von Frey试验评估伤害感受行为。此外，本研究通过血浆外渗（伊文思蓝试验）和多形核中性粒细胞的关节内浸润（髓过氧化物酶定量）来测量TMJ炎症。结果显示，遭受TMJ炎症的大鼠进入EPM开放臂的次数减少，在明暗箱试验中在亮区停留的时间和转换次数减少。此外，用作运动活动指标的EPM封闭臂中的进入次数在各处理组之间没有变化。此外，在整个炎症过程中，关节组织的机械敏感性增加，血浆外渗增加，同时TMJ滑液中的髓过氧化物酶增加。我们的结果表明，CFA诱导的颞下颌关节炎症在大鼠中产生了焦虑样行为，并在炎症的不同时期诱导了伤害感受行为。
