Lalor P A, Morrison W I, Goddeeris B M, Jack R M, Black S J
Vet Immunol Immunopathol. 1986 Sep;13(1-2):121-40. doi: 10.1016/0165-2427(86)90054-1.
Monoclonal antibodies were produced against bovine lymphoid cells. The reactivities of the antibodies for membrane determinants were examined on both cell suspensions and cryostat tissue sections prepared from bovine blood, thymus, spleen and lymph nodes. The antibodies were putatively grouped into sets which reacted with monomorphic and polymorphic determinants associated with bovine class I and class II major histocompatibility complex (MHC) antigens (MAbs P12 and P3, and R1 and P2 respectively), or associated with differentiation antigens expressed on T cells and monocytes (MAb P5) or exclusively on monocytes (MAb P8). The antibodies were used to identify the surface phenotypes of cells which stimulate (R1+ P5+ P8+) and proliferate (R1- P5+ P8-) in the bovine mixed leukocyte cultures, and cells which proliferate in response to the mitogen, concanavalin A (R1- P5+).
制备了针对牛淋巴细胞的单克隆抗体。在由牛血液、胸腺、脾脏和淋巴结制备的细胞悬液和低温恒温器组织切片上检测了这些抗体对膜决定簇的反应性。这些抗体被假定分为几组，分别与与牛I类和II类主要组织相容性复合体（MHC）抗原相关的单态性和多态性决定簇反应（分别为单克隆抗体P12和P3，以及R1和P2），或与T细胞和单核细胞上表达的分化抗原相关（单克隆抗体P5）或仅与单核细胞上表达的分化抗原相关（单克隆抗体P8）。这些抗体用于鉴定在牛混合淋巴细胞培养物中刺激（R1 + P5 + P8 +）和增殖（R1 - P5 + P8 -）的细胞以及对有丝分裂原刀豆球蛋白A有反应而增殖的细胞（R1 - P5 +）的表面表型。
