Emery D L, Puri N K, Dufty J H, Gorrell M D, Brandon M R
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic Australia.
Vet Immunol Immunopathol. 1987 Nov;16(3-4):215-34. doi: 10.1016/0165-2427(87)90019-5.
Monoclonal antibodies (MAbs) reacting with bovine (2) ovine (3), murine (1) or human (1) Class II MHC antigens were examined for reactivity with bovine peripheral blood leucocytes (PBL) and lymph node cells (LNC) by immunofluorescence, immunoprecipitation and the capacity to inhibit mixed lymphocyte responses (MLR), lectin- and antigen-induced blastogenesis. The 6 MAbs identified comparable percentages of Class II positive lymphocytes in PBL (40.8 to 54.2%) and LNC (6 to 11.5%) regardless of BoLA-A phenotype. Immunohistological staining of Class II MAb was localized principally to the lymphoid follicles in lymph nodes and to isolated epithelial reticular cells in the thymus. The anti-Class II MAb immunoprecipitated alpha- and beta- chains of 26-29K and 32-34K, respectively. These MAb inhibited proliferative responses in the MLR by between 25 and 74%, and diminished blastogenesis induced by specific antigens (purified protein derivative + PPD and ovalbumin) and B-lymphocyte mitogens (PPD, lipopolysaccharide and dextran sulphate) by between 45 and 75%, regardless of BoLA-A phenotype. In contrast, proliferation in response to concanavalin A and phytohaemagglutinin were unaffected by the anti- Class II MAb. Similarly these MAb did not affect lysis by cytotoxic T-lymphocytes, the activity of which was depressed by anti-Class I MAbs and monospecific alloantisera.
通过免疫荧光、免疫沉淀以及抑制混合淋巴细胞反应（MLR）、凝集素和抗原诱导的细胞增殖的能力，检测了与牛（2种）、羊（3种）、鼠（1种）或人（1种）II类主要组织相容性复合体（MHC）抗原发生反应的单克隆抗体（MAb）与牛外周血白细胞（PBL）和淋巴结细胞（LNC）的反应性。这6种MAb在PBL（40.8%至54.2%）和LNC（6%至11.5%）中鉴定出相当比例的II类阳性淋巴细胞，而与牛白细胞抗原A（BoLA - A）表型无关。II类MAb的免疫组织化学染色主要定位于淋巴结的淋巴滤泡和胸腺中孤立的上皮网状细胞。抗II类MAb分别免疫沉淀出26 - 29K的α链和32 - 34K的β链。这些MAb在MLR中抑制增殖反应的程度为25%至74%，并且使由特定抗原（纯化蛋白衍生物+ PPD和卵清蛋白）和B淋巴细胞有丝分裂原（PPD、脂多糖和硫酸葡聚糖）诱导的细胞增殖减少45%至75%，与BoLA - A表型无关。相比之下，抗II类MAb不影响对刀豆球蛋白A和植物血凝素的增殖反应。同样，这些MAb不影响细胞毒性T淋巴细胞的裂解作用，而抗I类MAb和单特异性同种抗血清可抑制其活性。
