Ni Songshi, Xu Liqin, Huang Jianfei, Feng Jian, Zhu Huijun, Wang Gui, Wang Xudong
Department of Respiratory Medicine, Affiliated Hospital of Nantong University Nantong, Jiangsu 226001, China.
Int J Clin Exp Pathol. 2013 Nov 15;6(12):2887-95. eCollection 2013.
The aim of this study was to investigate the expression of Zonula Occludens-1 (ZO-1) and its potential value as prognostic indicator of survival in patients with primary non-small cell lung cancer (NSCLC).
Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry with tissue microarrays were used to characterize the expression of the ZO-1 mRNA and protein in NSCLC. The correlation of ZO-1 expression with clinical characteristics and prognosis was determined by statistical analysis.
The ZO-1 mRNA and protein levels were significantly lower in NSCLC tissue compared with corresponding peritumoral tissue (P<0.05). ZO-1 protein expression in NSCLC was related to age (P=0.042) and 5-year survival (P<0.001). Kaplan-Meier survival and Cox regression analyses revealed that low ZO-1 expression (P<0.001) and later stage grouping by TNM (P=0.031) were independent factors predicting poor prognosis for patients with NSCLC.
Our findings provide the first evidence that high expression of ZO-1 is associated with good prognosis in NSCLC.
本研究旨在探讨闭合蛋白1（ZO-1）的表达及其作为原发性非小细胞肺癌（NSCLC）患者生存预后指标的潜在价值。
采用定量实时聚合酶链反应（qRT-PCR）和组织芯片免疫组化法，对NSCLC组织中ZO-1 mRNA和蛋白的表达进行特征分析。通过统计分析确定ZO-1表达与临床特征及预后的相关性。
与相应的癌旁组织相比，NSCLC组织中ZO-1 mRNA和蛋白水平显著降低（P<0.05）。NSCLC中ZO-1蛋白表达与年龄（P=0.042）和5年生存率（P<0.001）相关。Kaplan-Meier生存分析和Cox回归分析显示，ZO-1低表达（P<0.001）和TNM分期较晚（P=0.031）是预测NSCLC患者预后不良的独立因素。
我们的研究结果首次证明，ZO-1高表达与NSCLC的良好预后相关。
