First Department of Respiratory Medicine, Nanjing Chest Hospital, Nanjing 210029, PR China.
Oncol Rep. 2012 Apr;27(4):1072-8. doi: 10.3892/or.2011.1605. Epub 2011 Dec 21.
Caveolin-1 (cav-1) has been implicated in the development of human cancers. However, the distribution of cav-1 in non-small cell lung cancer (NSCLC) and its significance require further study. Real-time PCR and Western blot assays were performed to detect cav-1 mRNA and protein levels in tumor tissues (TT) and matched tumor-free tissues (TF). The protein expression in 115 paraffin-embedded blocks was examined by immunohistochemical staining (IHC). Correlations between cav-1 mRNA and protein expression by IHC and clinicopathological features were statistically evaluated. For the 136 patients examined, the levels of cav-1 mRNA and protein expression were significantly lower in lung TT compared to matched TF (P<0.05). High cav-1 expression was detected in 60 of 115 (52.2%) NSCLC tissues and this level was significantly lower than cav-1 expression in non-cancerous lung tissues (15 of 19, 78.9%, P<0.05). Up-regulation of cav-1 mRNA expression in lung adenocarcinoma (AC) (29.7%) was higher than that observed in lung squamous cell carcinoma (SCC) (15.8%). Statistical analysis of the correlation between cav-1 protein expression and clinical features showed a statistical association with poorer N-stage (P=0.032) and higher pathological TNM stage (P=0.012) in lung AC patients, that was not found in lung SCC patients. Moreover, lung AC patients with higher cav-1 expression showed significantly shorter life-spans than those with lower cav-1 expression (P=0.032, log-rank test). The levels of cav-1 mRNA and protein expression were significantly lower in lung cancers when compared to matched TF or non-cancerous lung tissues. The higher protein expression correlated with the advanced pathological stage and shorter survival rates in lung AC patients.
窖蛋白-1（cav-1）与人类癌症的发生发展有关。然而，cav-1 在非小细胞肺癌（NSCLC）中的分布及其意义仍需进一步研究。本研究采用实时 PCR 和 Western blot 法检测肿瘤组织（TT）和配对的无肿瘤组织（TF）中 cav-1 mRNA 和蛋白水平，应用免疫组织化学染色（IHC）检测 115 例石蜡包埋组织块中 cav-1 的蛋白表达，并对 cav-1 mRNA 和蛋白表达与临床病理特征的相关性进行统计学分析。在 136 例患者中，与配对 TF 相比，肺 TT 中 cav-1 mRNA 和蛋白表达水平显著降低（P<0.05）。在 115 例 NSCLC 组织中，60 例（52.2%）检测到 cav-1 高表达，显著低于非癌性肺组织中的表达（19 例中的 15 例，78.9%，P<0.05）。肺腺癌（AC）中 cav-1 mRNA 表达上调（29.7%）高于肺鳞癌（SCC）（15.8%）。cav-1 蛋白表达与临床特征的相关性分析显示，在肺 AC 患者中，cav-1 蛋白表达与较差的 N 分期（P=0.032）和较高的病理 TNM 分期（P=0.012）显著相关，但在肺 SCC 患者中未发现这种相关性。此外，肺 AC 患者中 cav-1 表达较高者的总生存期显著短于表达较低者（P=0.032，log-rank 检验）。与配对 TF 或非癌性肺组织相比，肺癌组织中 cav-1 mRNA 和蛋白表达水平显著降低。蛋白表达水平较高与肺 AC 患者的病理分期较晚和生存率降低相关。
